| 响应曲面法优化鸡冠中透明质酸提取工艺条件 |
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| 引用本文： | 郭瑞琳,王鑫,孟颖,魏静,许丹丹,宋天雪.响应曲面法优化鸡冠中透明质酸提取工艺条件[J].哈尔滨商业大学学报(自然科学版),2014(3):345-350. |
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| 作者姓名： | 郭瑞琳 王鑫 孟颖 魏静 许丹丹 宋天雪 |
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| 作者单位： | 哈尔滨商业大学食品工程学院省高校食品科学与工程重点实验室,哈尔滨150076 |
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| 基金项目： | 哈尔滨商业大学大学生创新训练项目（201310240092）. |
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| 摘 要： | 对乙醇浸泡鸡冠并提取其中透明质酸的方法进行研究,通过单因素和相应曲面实验考查了提取时间、乙醇体积分数、料液比对透明质酸得率的影响,确定了乙醇处理鸡冠中透明质酸的最佳工艺条件为:提取时间为25.70 h、乙醇体积分数90.00%、料液比100∶177(g/mL),在此条件下制备透明质酸的得率为27.31%.通过对该物质进行紫外光谱检测,显示该提取物质与标准品出峰位置一致,确定该提取物质是透明质酸.
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| 关 键 词： | 鸡冠 透明质酸 响应曲面法 乙醇 |
Optimization of process conditions for extraction of hyaluronic acid by response surface methodology |
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| GUO Rui-lin,WANG Xin,MENG Ying,WEI Jing,XU Dan-dan,SONG Tian-xue.Optimization of process conditions for extraction of hyaluronic acid by response surface methodology[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2014(3):345-350. |
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| Authors: | GUO Rui-lin WANG Xin MENG Ying WEI Jing XU Dan-dan SONG Tian-xue |
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| Institution: | ( Key Laboratory of Food Science and Engineering, School of Food Engineering, Harbin University of Commerce, Harbin 150076, China) |
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| Abstract: | Fresh cockscomb was used as the raw material and the hyaluronic acid extraction yield as the target to withdraw the method by ethanol precipitation .The ranges of all affecting factors were determined by single factor experiment and Box -Behnken center combination experimental design used to set up two times multiterm mathematical model .The result indi-cated that the data results significantly and the optimum conditions of ethanol precipitation was obtained as follows： the extraction time was 25.70 h, hyaluronic acid extraction yield was 90.00%,solid-liquid ratio was 100∶177 （ g/mL） and the hyaluronic acid extraction yield was 23 .73%.Determine the extraction of substances through the UV spectrum detection of hyaluronic acid . |
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| Keywords: | cockscomb hyaluronic acid response surface methodology alcohol |
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