激素和添加物对蝴蝶兰组培快繁的影响

以蝴蝶兰人工授粉的果荚种胚和组培苗的叶片、茎尖为外植体,进行组织培养快速繁殖研究.结果表明,果荚种胚是最好的外植体材料,1个果荚可培育出上万个原球茎.种胚诱导原球茎最佳激素组合为:6-BA 0.3mg/L+NAA 0.2 mg/L;原球茎增殖最佳激素组合为:6-BA 0.5 mg/L+NAA 0.2 mg/L;2%蔗糖和10%香蕉泥可以显著促进原球茎增殖;原球茎分化最佳激素组合为:6-BA 1.0 mg/L+NAA 0.2 mg/L;诱导生根IBA 0.5mg/L最佳;炼苗后移栽苗成活率达95%以上.优化后的蝴蝶兰组织培养条件,可为蝴蝶兰快速繁殖工厂化生产提供参考.

The Optimizations of Protocorm Induction Route and Culture Process in Tissue Culture of Phalaenopsis amabilis

The study of tissue culture of Phalae.nopsis amabilis used seed pod of artificial pollination and leaves, stem apex of tubing shoots as explants. The results showed that pod＇ s seed embryo is the best explant material, a pod can cultivate tens of thousands of protocorms, the optimal combinations of hormone concentration in protocorm induction was 6-BA 0.3 mg/L ＋ NAA 0.2 mg/L; protocorm proliferation was 6-BA 0.5 mg/L ＋ NAA 0.2 mg/L, and 2% sucrose combined with 10% banana mud could significantly promote the proliferation of protocorm. The optimal combination of hormone concentration in protocorm differentiation was 6-BA 1.0 mg/L ＋ NAA 0.2 rag/L; 0.5mg/L IBA was the best hormone concentration in root induction. The survival rate of transplantation after hardening tubing shoots was more than 95%. The optimum technological conditions of tissue culture of Phalaenopsis could provide references for the industrialized production of rapid propagation of Phalaenopsis.