| 抗基因工程人rIFN-γ杂交瘤细胞株的建立 |
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| 引用本文： | 丛进阳,丁宝玉,沈金玉.抗基因工程人rIFN-γ杂交瘤细胞株的建立[J].清华大学学报(自然科学版),1992(3). |
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| 作者姓名： | 丛进阳 丁宝玉 沈金玉 |
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| 作者单位： | 化学工程系
(丛进阳，丁宝玉)，化学工程系(沈金玉) |
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| 摘 要： | 用杂交瘤技术将基因工程人γ－干扰素（ｒＩＦＮ－γ）免疫的Ｂ淋巴细胞与ｓｐ２／０鼠骨髓瘤细胞融合，建立了 ３株抗 ｒＩＦＮ－γ杂交瘤细胞株。这些细胞株经两年多的传代培养仍保持稳定分泌抗体的能力，并与ｒＩＦＮ－γ及新型γ－干扰素呈特异性反应，可用于亲和层析纯化γ－干扰素．
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| 关 键 词： | 基因工程人γ-干扰素 单克隆抗体 杂交瘤细胞 |
Hybridoma cell lines secreting monoclonal antibodies against recombinant human interferon--r |
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| Cong Jinyang,Ding Baoyu,shen Jinyu.Hybridoma cell lines secreting monoclonal antibodies against recombinant human interferon--r[J].Journal of Tsinghua University(Science and Technology),1992(3). |
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| Authors: | Cong Jinyang Ding Baoyu shen Jinyu |
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| Institution: | Cong Jinyang,Ding Baoyu,shen Jinyu Department of Chemical Engineering |
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| Abstract: | Three hybridoma cell lines secreting monoclonal antibodies against recombinant human interferon-gamma (rIFN-r) have been established by fusion of myoloma cells sp2/0 with B lymphocytes derived from immunized BALB/c mice. All these cell lines remain stable in secreting high titer of monoclonal antibodies during more than two years' continuous culture. The monoclonal antibodies are specific for binding both rIFN-r and new types of interferon-gamma. They are expected to be suitable:for immunoaffinity purification of interferon-gamma. |
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| Keywords: | recombinant human interferon-gamma monoclonal antibodies hybridoma cell |
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