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| 藏羚羊5个微卫星标记的多态性分析 | |
| 引用本文： | 周智红,郭松长,马建滨,徐永涛,谭春敏,都玉蓉.藏羚羊5个微卫星标记的多态性分析[J].青海师范大学学报(自然科学版),2014(2):36-39. |
| 作者姓名： | 周智红 郭松长 马建滨 徐永涛 谭春敏 都玉蓉 |
| 作者单位： | 1. 青海师范大学生命与地理科学学院,青海 西宁,810008 2. 中国科学院高原生物适应与进化重点实验室,青海 西宁,810001 3. 青海师范大学生命与地理科学学院，青海 西宁 810008; 中国科学院高原生物适应与进化重点实验室，青海 西宁 810001 |
| 基金项目： | 青海省应用基础研究计划(2013-Z -751 |
| 摘 要： | 藏羚羊是我国青藏高原的特有物种.为分析藏羚羊独特的遗传变异特征和核DNA遗传多样性,以5个微卫星标记对50只藏羚羊的等位基因多态性进行了研究.研究结果表明:5个微卫星标记共检测到92个等位基因,每个标记的平均等位基因数为18.4个(在16~23之间),平均有效等位基因数为11.1;5个微卫星标记的多态信息含量均在0.8573以上,为高度多态标记,其中L03标记的多态信息含量最高,达0.9372;各标记的观测杂合度在0.4898~0.9091之间,期望杂合度在0.8770~0.9504之间,平均期望杂合度为0.8990,属于高度杂合标记,遗传变异丰富.这些筛选出的多态性微卫星标记可应用于藏羚羊遗传多样性、遗传结构分析及遗传图谱的构建等工作. |
| 关 键 词： | 藏羚羊 微卫星 多态性 |
Genetic Diversity of Endangered Tibetan Antelope Revealed by Microsatellite Markers |
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| ZHOU Zhi-hong,GUO Song-chang,MA Jian-bin,XU Yong-tao,TAN Chun-min,DU Yu-rong.Genetic Diversity of Endangered Tibetan Antelope Revealed by Microsatellite Markers[J].Journal of Qinghai Normal University(Natural Science Edition),2014(2):36-39. | |
| Authors: | ZHOU Zhi-hong GUO Song-chang MA Jian-bin XU Yong-tao TAN Chun-min DU Yu-rong |
| Institution: | ZHOU Zhi-hong ,GUO Song-chang , MA Jian-bin ,XU Yong-tao , TAN Chun-min , DU Yu-rong (1. School of Life and Geography Science, Qinghai Normal University, Xining 810008, China; 2. Key Laboratory of Evolution and Adaptation of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China) |
| Abstract: | Tibetan antelope（pantholops hodgsonii）,also known as the chiru,is endemic to the Qinghai-Tibet Plateau.A total of 50 individual of Tibetan antelope were analyzed using five microsatellite markers.With an average of 18.4 alleles per locus（ranging from 16 to 23）,92 alleles were identified at 5 microsatellite loci.The average effective number of allele was 11.1,and the polymorphism information content（PIC）of the 5 markers were more than 0.8573,which indicated a high polymorphism.With an value of 0.9372,the highest PIC was observed at locus L03.The observed heterozygote（Ho）of the 5 markers ranged from 0.4898 to 0.9091,and the expected heterozygote（He）varied from 0.8770 to 0.9504（average 0.8990）.The results revealed a high level of genetic variation at 5 loci,which suggested that those microsatellite markers could be applied in studies of genetic diversity,genetic constructure and construction of the genetic map in Tibetan antelope. |
| Keywords: | Tibetan antelope microsatellite polymorphism |
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