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基于含蜡状芽孢杆菌的生物刺激-生物强化联合体系降解石油污染物
引用本文:邓振山,高飞,刘玉珍,魏婷婷,余天飞,王华,刘鑫垚,边丹.基于含蜡状芽孢杆菌的生物刺激-生物强化联合体系降解石油污染物[J].南华大学学报(自然科学版),2018,32(2):19-25.
作者姓名:邓振山  高飞  刘玉珍  魏婷婷  余天飞  王华  刘鑫垚  边丹
作者单位:延安大学生命科学学院
基金项目:陕西省科技统筹创新工程项目(2012KTZB03-02-03);陕西省教育厅服务地方专项计划项目(16JE029); 陕西省协同创新计划项目 (2015XT-34);陕西省2016年科技统筹创新工程三批计划(2016ZCKJ3-106)
摘    要:为了探讨生物刺激、生物强化及其联合体系对石油污染物的去除能力,选择最佳的修复策略,更好地发挥微生物修复的优势.以一株蜡状芽孢杆菌石油降解菌为例,以有机营养物、无机营养物和混合营养物为底物,进行生物刺激、生物强化试验及联合降解试验.结果显示,不同修复方式在不同时段的降解效果不同,生物刺激方式在第5天时混合营养物降解效果最好,降解率达到40.98%,在10 d不同营养物的降解率存在明显的差距,而在处理20 d后,降解率基本一致,达到80%.生物强化的处理结果中,以混合营养物为底物时,表现出了优异的降解效果,降解率达到90.23%.研究表明:不同时期不同的修复策略有表现出了对原油的不同去除能力,也为实践中选择合理的修复方式提供科学的理论指导.

关 键 词:生物刺激  生物强化  蜡状芽孢杆菌  石油污染  生物修复
收稿时间:2018/2/27 0:00:00

Biodegradation of Petroleum Pollutants Based Biostimulation-bioaugmentation System with Bacillus Cereus
DENG Zhen-shan,GAO Fei,LIU Yu-zhen,WEI Ting-ting,YU Tian-fei,WANG Hu,LIU Xin-yao and BIAN Dan.Biodegradation of Petroleum Pollutants Based Biostimulation-bioaugmentation System with Bacillus Cereus[J].Journal of Nanhua University:Science and Technology,2018,32(2):19-25.
Authors:DENG Zhen-shan  GAO Fei  LIU Yu-zhen  WEI Ting-ting  YU Tian-fei  WANG Hu  LIU Xin-yao and BIAN Dan
Institution:College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China,College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China and College of Life Sciences,Yan''an University,Yan''an,Shanxi 716000,China
Abstract:In order to investigate the removal ability of biological stimulation,biological enhancement and its combined system to remove petroleum pollutants,the best repair strategy was chosen to better utilize the advantages of microbial remediation.Taking an oil degrading Bacillus cereus as an example,the biological stimulation,biological enhancement test and combined degradation test were carried out on the basis of organic nutrients,inorganic nutrients and mixed nutrients.Different repair methods in different periods had different degradation effect,and the biological stimulation had the best degradation effect on the fifth day when the degradation rate of the mixed nurients reached 40.98%,on the 10th day the degradation rate of different nurients had obvious differences,and on the 20th day,the degradation rate reached 80% which was consistent.In the treatment of bioaugmentation,when mixed nutrients were used as substrates,the degradation rate was excellent,and the degradation rate was 90.23%.Different periods and different repair strategies showed different removal ability of crude oil,which provided some theoretical significance for selecting reasonable repair methods in practice.
Keywords:biostimulation  bioaugmentation  bacillus cereus  petroleum  pollutants  bioremediation
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