丝素包埋SPA成膜的理化性能和生物效应研究

研究将丝素溶液包埋金黄色葡萄球菌A蛋白(staphylococcal protein A)(简称A蛋白或SPA ),制成不溶性SPA丝素膜.用粘附生长因子RGD(序列为GLY-ARG-GLY-ASP-SER-PRO-L YS)的抗体IgG结合IgG到该丝素膜的表面,再将RGD结合到不溶性SPA丝素膜表面RGD抗体IgG 上,制备成RGD-IgG-SPA丝素膜.用酶联免疫吸附试验(简称ELISA)方法,证明RGD-IgG -SPA丝素膜是稳定的.种植培养血管内皮细胞(vascular endothelial cell,简称EC细胞) 于该改性丝素膜的表面,用四甲基偶氮唑盐比色方法(简称MTT法)检测细胞的生长,证明改性丝素膜能有效地促进EC细胞的生长.

A Study on the Effect of Growth of Endothelial Cells upon Biological Modifications of Silk

Immobilized with silk solution, staphylococcal protein A (SPA) can be made into an insoluble silk membrane and then adsorbed to antiRGD antibody IgG to an SPA silk membrane, then adsorbed RGD (synthetic peptide, GLY-ARG-GLY-ASP-SER-PRO-LYS) which is adhesive and growth factor to IgG-SPA silk membrane(namely RGD-IgG-SPA silk membrane). The activity of SPA immobilized silk membrane was investigated by the enzyme-linked immunosorbent assay(ELISA). The results demonstrated that the stability of SPA immobilized silk membrane has been greatly improved. Vascular endothelial cells were seeded onto the modified silk membrane. Effectiveness of the absorbance in the survival of cells was verified by tetrazolium-based assay (MTT). It demonstrated that RGD adsorbed IgG-SPA immobilized silk membrane could keep its bioactivity functionally.