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| 苏铁蕨多糖的提取及其组分分析 | |
| 引用本文： | 樊亚鸣,陶文琴,高绍中,黄晓兰,陈怡莎,艾小红,陈永亨.苏铁蕨多糖的提取及其组分分析[J].广州大学学报(自然科学版),2008,7(6). |
| 作者姓名： | 樊亚鸣 陶文琴 高绍中 黄晓兰 陈怡莎 艾小红 陈永亨 |
| 作者单位： | 1. 广州大学化学化工学院,广东广州,510006 2. 广州大学生命科学学院,广东广州,510006 3. 中国广州分析测试中心,广东广州,510275 4. 广州大学环境科学与工程学院,广东广州,510006 |
| 基金项目： | 国家星火计划项目 ， 广东省自然科学基金资助项目 ， 广东省科技汁划项日 |
| 摘 要： | 采用低温-微波工艺提取苏铁蕨Brainia insignis（Hook．）J．Sm．]的木质根状茎部分，以95％乙醇、水为溶剂分步提取，获苏铁蕨粗多糖，经纯化为精制多糖．以凝胶过滤色谱法（GFC）测定苏铁蕨多糖的数量及分子量．结果：苏铁蕨中含1种多糖，其数均分子量为31514g·mol^-1，重均分子量为35034g·mol^-1；多糖经酸解成单糖并衍生为乙酰化糖肟，对照乙酰化的标样采用气相色谱法（GC）测定其中含5种单糖组分，其相对含量：葡萄糖88．816％；半乳糖4．631％；阿拉伯糖3．314％；木糖2．085％；甘露糖1．154％． |
| 关 键 词： | 苏铁蕨 多糖 单糖组分 |
Purification and analysis of the Brainia insignis(Hook.) J.Sm.polysaccharides |
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| FAN Ya-ming,TAO Wen-qin,GAO Shao-zhong,HUANG Xiao-lan,CHEN Yi-sha,AI Xiao-hong,CHEN Yong-heng.Purification and analysis of the Brainia insignis(Hook.) J.Sm.polysaccharides[J].Journal og Guangzhou University:Natural Science Edition,2008,7(6). | |
| Authors: | FAN Ya-ming TAO Wen-qin GAO Shao-zhong HUANG Xiao-lan CHEN Yi-sha AI Xiao-hong CHEN Yong-heng |
| Institution: | FAN Ya-ming1,TAO Wen-qin2,GAO Shao-zhong1,HUANG Xiao-lan3,CHEN Yi-sha1,AI Xiao-hong1,CHEN Yong-heng4(1.School of Chemistry , Chemical Engineering,Guangzhou University,Guangzhou 510006,China,2.School of Life Sciences,3.China Guangzhou Analysis , Test Center,Guangzhou 510070,4.School of Environmental Science , Engineering,China) |
| Abstract: | The upstanding part of xylem caudex for Brainia insignis(Hook.) J.Sm.was respectively extracted by 95% ethanol and distilled water in lower temperature and under mild microwave irradiation,and the crude Brainia insignis polysaccharide was obtained and then purified further to the refined polysaccharide.Testing the number and molecular weight of Brainia insignis polysaccharide by Gel Filtration Chromatography(GFC),the result is that there is only one kind of Brainia insignis polysaccharide and its Mn is 31 5... |
| Keywords: | Brainia insignis(Hook ) J Sm polysaccharide the components of monosaccharides |
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