| 含硫树脂的制备及在谷胱甘肽分离中的应用 |
| |
| 引用本文： | 赵睿,王满意,周鑫,谭天伟.含硫树脂的制备及在谷胱甘肽分离中的应用[J].北京化工大学学报(自然科学版),2007,34(3):304-307. |
| |
| 作者姓名： | 赵睿 王满意 周鑫 谭天伟 |
| |
| 作者单位： | 北京化工大学1.生命科学与技术学院；2.北京市生物加工过程重点实验室， 北京 100029 |
| |
| 摘 要： | 利用大孔苯乙烯树脂改性合成含硫树脂，考察了修饰条件对谷胱甘肽吸附的影响。并对其在谷胱甘肽分离中的应用进行了研究。结果表明采用pH3.0的缓冲液进行吸附，自制的含硫树脂最大吸附容量可达22mg/g湿树脂，选择质量浓度为0.5%的NaOH溶液洗脱GSH，解吸率可达70%以上。采用真实发酵液进行分离纯化，收率为35%，纯度可达90%以上。
|
| 关 键 词： | 含硫树脂 修饰 谷胱甘肽 含硫树脂 修饰 谷胱甘肽 |
| 收稿时间： | 2006-12-19 |
| 修稿时间： | 2006-12-19 |
The preparation of resin containing sulf-group and their application in glutathione separation |
| |
| ZHAO Rui,WANG ManYi,ZHOU Xin,TAN TianWei.The preparation of resin containing sulf-group and their application in glutathione separation[J].Journal of Beijing University of Chemical Technology,2007,34(3):304-307. |
| |
| Authors: | ZHAO Rui WANG ManYi ZHOU Xin TAN TianWei |
| |
| Institution: | 1.Beijing Key Laboratory of Bioprocess;2.College of Life Science and Technology,Beijing University of Chemical Technology, Beijing 100029, China |
| |
| Abstract: | Chloromethylated styrene-divinylbenzene resin has been modified with thiourea under a variety of conditions and the adsorption and desorption of glutathione on the modified resins has been investigated. The optimal pH for adsorption was found to be 3.0, giving a maximum adsorption capacity of glutathione of 22 mg/g wet resin. Recovery of glutathione reached 70 % when 0.5 96 - 1.5 % NaOH solution was employed for desorption. Chromatography experiments indicated that GSH could be successfully purified from clarified broth, and that the resins have potential applications in GSH separation. |
| |
| Keywords: | sulf-resin modified glutathione |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《北京化工大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《北京化工大学学报(自然科学版)》下载免费的PDF全文 |
