| 溶菌酶脂质体的制备及其对生物膜的剥离作用 |
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| 引用本文： | 徐冉,王海峰,李风亭.溶菌酶脂质体的制备及其对生物膜的剥离作用[J].同济大学学报(自然科学版),2011,39(1):90-93. |
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| 作者姓名： | 徐冉 王海峰 李风亭 |
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| 作者单位： | 同济大学,污染控制与资源化研究国家重点实验室,上海,200092 |
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| 基金项目： | “十一五”国家科技支撑计划(2009BAC62B00,2009BAC62B03); 高等学校博士学科点专项科研基金(200802471044); 中国与乌克兰政府间科技合作项目(CU08-20); 科技部国际科技合作项目(2009DFA90740) |
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| 摘 要： | 采用逆向蒸发法制备了稳定的溶菌酶脂质体.在不锈钢表面培养出稳定生物膜后,分别利用溶菌酶和溶菌酶脂质体对其进行剥离.运用Zeta电位仪、扫描电子显微镜(SEM)和红外光谱(FT-IR)对脂质体和生物膜进行表征.结果表明制备的脂质体平均粒径为80~100 nm,包封率为82.4%.相同浓度下溶菌酶及其脂质体对混合菌种形成的生物膜剥离效率分别达到62.4%和86.5%.溶菌酶脂质体在24h内对生物膜和水体中微生物去除率分别达到89.6%和99.6%.因此,溶菌酶脂质体能够有效控制不锈钢表面生物膜污染风险.
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| 关 键 词： | 脂质体 溶菌酶 生物膜剥离 微生物 |
| 收稿时间： | 2009/10/15 0:00:00 |
| 修稿时间： | 12/5/2010 9:01:25 PM |
Lysozyme liposome Synthesis and Its Capability in Biofilm Removal |
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| XU Ran,WANG Haifeng and LI Fengting.Lysozyme liposome Synthesis and Its Capability in Biofilm Removal[J].Journal of Tongji University(Natural Science),2011,39(1):90-93. |
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| Authors: | XU Ran WANG Haifeng and LI Fengting |
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| Institution: | XU Ran,WANG Haifeng,LI Fengting(State Key Laboratory of Pollution Control and Resource Reuse,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China) |
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| Abstract: | Stable lysozyme-liposomes were synthesized via reverse-phase evaporation.Lysozyme and its liposomes were separately used to remove the stable biofilms cultivated on stainless steel surface.The liposomes and biofilms were characterized by Zeta potential analyzer,transmission electron microscope(TEM),scanning electron microscope(SEM)and Fourier transform infrared(FT-IR)spectrometer.The experimental results show that the liposomes are of the average diameter of 80~100 nm and an average entrapment efficiency of... |
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| Keywords: | liposome lysozyme biofilm removal microorganism |
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