喹唑啉分子印迹聚合物微球的合成及识别性能

以2,4-二氯-6,7-二甲氧基喹唑啉(DCQAL)为模板分子,采用单步溶胀聚合法制得了分子印迹聚合物微球(MIPM),研究了功能单体、模板分子与功能单体的摩尔比、交联剂用量及吸附液添加剂等对MIPM识别性能的影响.结果表明:对于碱性的DCQAL,采用酸性功能单体制得的MIPM可与之形成较强的氢键从而显示出较好的识别性能,以4-氯-6,7-二甲氧基喹唑啉或2,3-二甲基喹喔啉为竞争分子,其分离因子分别达1.83和2.02;增加交联剂的用量可增强印迹孔穴的稳定性,从而提高识别能力;在一定范围内增大模板分子与功能单体的摩尔比,能使更大比例的功能单体在MIPM中形成有序的排列,使MIPM呈现更好的识别效果;吸附液中添加少量的三乙胺(含量小于0.1%)可减弱MIPM的非特异性吸附,从而提高其识别能力.

Synthesis and Recognition Capabilities of Quinazoline Molecularly-Imprinted Polymeric Microspheres

Molecularly-imprinted polymeric microspheres(MIPM) were prepared via a one-step swelling and polymerization method,with 2,4-dichloro-6,7-dimethoxyquinazoline(DCQAL) as the template molecule.The effects of the functional monomer,the molar ratio of the template molecule to the functional monomer,the crosslinker do-sage and the additive of adsorption solution on the recognition capabilities of MIPM were then investigated.The results indicate that(1) the MIPM prepared using acidic functional monomers can form stronger hydrogen bond with basic DCQAL,thus resulting in good recognition capabilities for DCQAL,the separation factor is up to 1.83 or 2.02 when 4-chloro-6,7-dimethoxyquinazoline or 2,3-dimethylquinoxaline is chosen as the competitive molecule;(2) an increase in the crosslinker dosage may enhance the stability of the imprinted cavity of MIPM and thus improves the recognition capabilities of MIPM;(3) when increasing the molar ratio of the template molecule to the functional monomer,more functional monomers can be regularly arranged in MIPM to achieve better recognition capabilities;and(4) the addition of a small quantity of triethylamine(less than 0.1%) to the adsorption solution can decrease the non-specific adsorption of MIPM,thus improving the recognition capabilities.