| 不同方法处理的角膜脱细胞基质组织学生物特性的比较 |
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| 引用本文： | 刘晓霞,陈建苏,徐锦堂,郑佩娥,梁晓东.不同方法处理的角膜脱细胞基质组织学生物特性的比较[J].暨南大学学报,2007,28(2):164-167. |
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| 作者姓名： | 刘晓霞 陈建苏 徐锦堂 郑佩娥 梁晓东 |
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| 作者单位： | 1. 暨南大学医学院眼科研究室,广东,广州,510632
2. 暨南大学医学院病理研究室,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金
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广东省自然科学基金 |
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| 摘 要： | 目的:比较不同的角膜脱细胞基质方法,旨在选择最适合的脱细胞基质方法,制备成为组织工程角膜支架材料和角膜替代物的脱细胞基质.方法:分别用胰酶-曲拉通法、SDS-戊二醛法、胰酶-冷冻法等方法对角膜进行脱细胞处理,并对脱细胞彻底的基质进行力学特性、透光率的分析.结果:用胰酶-冷冻法获得的兔角膜全层和分层基质脱细胞最彻底,基质的结构未见明显破坏,脱细胞基质在生物力学特性上与正常角膜接近,透光率低于新鲜角膜.结论:用胰酶-冷冻法获得的脱细胞基质与自然角膜接近,可作为组织工程角膜支架材料和角膜替代物.
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| 关 键 词： | 角膜 角膜替代物 脱细胞基质 生物学性质 方法 脱细胞处理 正常角膜 脱细胞基质 组织学 生物特性 比较 biological characteristics matrix 生物力学特性 破坏 结构 全层 兔角膜 结果 分析 透光率 冷冻法 戊二醛法 通法 |
| 文章编号： | 1000-9965(2007)02-0164-04 |
| 修稿时间： | 11 30 2006 12:00AM |
Compararision of differently treated acculuar corneal matrix biological characteristics |
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| LIU Xiao-xia,CHEN Jian-su,XU Jin-tang,ZHENG Pei-e,LIANG Xiao-dong.Compararision of differently treated acculuar corneal matrix biological characteristics[J].Journal of Jinan University(Natural Science & Medicine Edition),2007,28(2):164-167. |
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| Authors: | LIU Xiao-xia CHEN Jian-su XU Jin-tang ZHENG Pei-e LIANG Xiao-dong |
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| Institution: | Department of Ophthalmology, Medical college, Jinan University, Guangzhan 510632, China |
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| Abstract: | Aim:To compare the method of different acculuar corneal matrix and study their biological characteristics. Methods: Fresh rabbit cornea were treated to obtain acculuar corneal matrix then were stained with HE and measure their characteristics.Results: There were no celluar components in ACCM and there were no distinct differences of mechanical characteristic between ACCM and cornea,the light trasmittance of ACCM was low compared with that of cornea.Conclusion:ACCM(trypsin-frozen method) can be used as a biological scaffold for tissue engineering and corneal substitute. |
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| Keywords: | cornea corneal substitute acculuar corneal matrix biological characteristic |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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