封闭式光生物反应器集胞藻6803光自养和混合营养培养比较

采用封闭式光生物反应器进行了蓝藻基因工程常用宿主系统集胞藻Synechocystis sp. PCC 6803的混合营养培养，并与光自养培养进行了比较，在两种培养方式下，集胞藻6803的饱和光强基本相同，都为5000lx，当入射光强为5000lx，初始葡萄糖浓度为1．74g/L时，混合营养生长在葡萄糖消耗完（69．5h)时的藻细胞密度为1．36g/L，叶绿素浓度为20．08mg/L，能量得率为16．7％，分别为同期光自养生长的3．8倍，2．3倍和2．6倍，这表明封闭式光生物反应器混合营养培养方式在促进集胞藻6803生长和光合色素合成及提高培养过程能量得率等方面都有显著作用。

Mixotrophic and Photoautotrophic Cultivation of Synechocystis sp. PCC 6803 in an Enclosed Photobioreactor

Compared with that of photoautotrophic cultivation, the characteristics of mixortophic cultivation of Synechocystis sp. PCC 6803 in an enclosed photobioreactor were investigated. The saturated level of light intensity of mixotrophic growth in the photobioreactor was 5 000 lx, which was the same as that of photoautotrophic growth. At incident light intensity 5 000 lx and initial glucose concentration 1.74g/L, after 69.5 h mixotrophic cultivation, 1.36g/L cell density, 20.08mg/L chlorophyll concentration and 16.7% energy efficiency were achieved, which were respectively 3.8 times, 2.3 times and 2.6 times as much as those in photoautotrophic cultivation. On the basis of above results, it was concluded that mixotrophic cultivation system had advantages over photoautotrophic cultivation system for Synechocystis sp. PCC 6803 on production of biomass, photosynthetic pigment and energy utilization.