Development of a hybrid scaffold and a bioreactor for cartilage regeneration |
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Authors: | Seung-Jae Lee In Hwan Lee Jeong Hun Park So-Jung Gwak Jong-Won Rhie Dong-Woo Cho Tae Jo Ko and Dong Sung Kim |
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Institution: | (1) Department of Biomedical Engineering, University of Alabama at Birmingham (UAB), Birmingham, AL 35294, USA;(2) Center for Nanoscale Materials and Biointegration (CNMB), Department of Physics, University of Alabama at Birmingham (UAB), Birmingham, AL 35294, USA; |
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Abstract: | We developed a hybrid scaffold and a bioreactor for cartilage regeneration. The hybrid scaffold was developed as combination of two components: a biodegradable framework and hydrogel-containing chondrocytes. We performed the MTT cell proliferation assay to compare the proliferation and viability of chondrocytes on three types of scaffolds: an alginate gel, the hybrid scaffold, and an alginate sponge. Cells were encapsulated in 2% agarose gel. The bioreactor consisted of a circulation system and a compression system. We performed dynamic cell culture on these agarose gels in the bioreactor for 3 days. |
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Keywords: | cartilage scaffold bioreactor alginate agarose |
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