| 钙羧酸指示剂与牛血清白蛋白作用的研究 |
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| 引用本文： | 黄发德,俞英,蒋雄.钙羧酸指示剂与牛血清白蛋白作用的研究[J].华南师范大学学报(自然科学版),2001(1):88-92. |
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| 作者姓名： | 黄发德 俞英 蒋雄 |
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| 作者单位： | 华南师范大学化学系 |
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| 摘 要： | 在pH4.47的缓冲溶液中，用分光光度法和平衡透析法研究钙羧酸指示剂（CCA）与牛血清白蛋白（BSA）的结合反应。研究发现，CAA与BSA主要是以分子间的疏水作用结合，该结合反应符合Pesavento提出的相分配模型，BSA对CCA的作用相类似于阳离子表面活性剂的作用，并探讨了实验条件对结合反应的影响。
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| 关 键 词： | 牛血清白蛋白 钙羧酸指示剂 分光光度法 平衡透析法 结合形式 结合力 结合数 |
| 文章编号： | 1000-5463(2001)01-0088-05 |
| 修稿时间： | 2000年6月27日 |
THE STUDY ON THE REACTION BETWEEN CALCONCARBOXYLIC ACID AND BOVINE SERUM ALBUMIN |
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| HUANG Fa-de,YU Ying,Jiang XIONG.THE STUDY ON THE REACTION BETWEEN CALCONCARBOXYLIC ACID AND BOVINE SERUM ALBUMIN[J].Journal of South China Normal University(Natural Science Edition),2001(1):88-92. |
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| Authors: | HUANG Fa-de YU Ying Jiang XIONG |
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| Abstract: | The interaction of Calconcarboxylic Acid(CCA) and bovine serum albumin(BSA) was investi- gated by using spectrophotometric method and equilibrium dialysis method in acidic solution(pH4.47). It is suggested that the hydrophobic force is the main binding force.The model of phase distribution is ap- propriate in the treatment of data obtained here. The effect of surface active agent to the interactional sys- tem is discussed. It is found that the effects of BSA on the spectrum of CCA were similar to that of cationic surfactant.The influences of experimental conditions on the interaction were also discussed. |
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| Keywords: | Calconarboxylic Acid Bovine Serum Albumin(BSA) spectrophotomety equlibrium dialy- sis method |
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