| 固相萃取高效液相色谱测定鱼腥草、甜茶中的糖 |
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| 引用本文： | 闫建荣,杨亚玲,刘谋盛.固相萃取高效液相色谱测定鱼腥草、甜茶中的糖[J].云南大学学报(自然科学版),2007,29(4):404-407. |
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| 作者姓名： | 闫建荣 杨亚玲 刘谋盛 |
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| 作者单位： | 1. 云南国防工业职业技术学院,云南,昆明,650224
2. 昆明理工大学,生命科学与技术学院,云南,昆明,650224 |
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| 摘 要： | 研究了高效液相色谱法测定鱼腥草和甜茶中的糖.样品中的糖提取液用Waters Sep-pak-C18固相萃取小柱预分离,以Waters carbohydrate高效糖柱为固定相,乙腈-水(体积比70:30)为流动相分离,ELSD2000蒸发光散射检测器检测,实现了8种水溶性单糖和二糖的同时测定.
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| 关 键 词： | 高效液相色谱法 糖 鱼腥草 甜茶 固相萃取 |
| 文章编号： | 0258-7971（2007）04-0404-04 |
| 修稿时间： | 2007-03-06 |
Study on sugars in Lithhocarpus polgstachrch Rehd and Houttuynia solid phase extraction and RP-HPLC |
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| YAN Jian-rong,YANG Ya-ling,LIU Mou-sheng.Study on sugars in Lithhocarpus polgstachrch Rehd and Houttuynia solid phase extraction and RP-HPLC[J].Journal of Yunnan University(Natural Sciences),2007,29(4):404-407. |
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| Authors: | YAN Jian-rong YANG Ya-ling LIU Mou-sheng |
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| Institution: | 1. Yunnan Institute of Technology, Kunming 650224, China; 2. College of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China |
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| Abstract: | Determination of sugars in Lithhocarpus polgstachrch Rehd and Houttuynia with HPLC and include the contents of water soluble sugars and the component of polysaccharides.The water soluble sugars chromatography were extracted with water by ultrasonic oscillation and by solid phase extraction with Sep-pak C18 cartridge.Sugars were separated on a Waters high performance carbohydrate column as solid phase,with V (acetonitrile):V (water)=70:30 as mobile phase,and detected with evaporative light-scatter detector.The method was applied to the determination of water soluble monosaccharides and polysaccharides at the same time. |
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| Keywords: | high performance liquid chromatography(HPLC) sugars Lithhocarpus polgstachrch Rehd Houttuynia solid phase extraction |
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