| 适合于MALDI-TOF质谱分析蛋白质及亚基的SDS-PAGE技术 |
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| 引用本文： | 方雪萍,黄河清.适合于MALDI-TOF质谱分析蛋白质及亚基的SDS-PAGE技术[J].厦门大学学报(自然科学版),2004,43(3):393-397. |
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| 作者姓名： | 方雪萍 黄河清 |
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| 作者单位： | 厦门大学生命科学学院,福建,厦门,361005 |
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| 基金项目： | 国家自然科学基金(40276033)，厦门科技项目 |
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| 摘 要： | 介绍适合于MALDI TOF质谱分析蛋白质及亚基的SDS PAGE技术,其主要特点是采用铜染色法在凝胶板上直接显色且采用有机溶剂萃取电泳纯的蛋白质和亚基,并用MALDI TOF质谱技术直接分析它们的分子量.本技术具有简便、快速和损失率小等优点,尤其适合于蛋白质组学、利用数据库分析蛋白质同源性和确定蛋白质种类等结构信息的研究,是一种较为理想的SDS PAGE和MALDI TOF质谱非在线联用分析技术.
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| 关 键 词： | 铜染 SDS-PAGE MALDI-TOF 亚基 分子量 |
| 文章编号： | 0438-0479(2004)03-0393-05 |
| 修稿时间： | 2003年6月26日 |
A Novel SDS-PAGE Method for Analyzing Proteins and Subunits with MALDI-TOF Mass Spectrometry |
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| FANG Xue-ping,HUANG He-qing.A Novel SDS-PAGE Method for Analyzing Proteins and Subunits with MALDI-TOF Mass Spectrometry[J].Journal of Xiamen University(Natural Science),2004,43(3):393-397. |
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| Authors: | FANG Xue-ping HUANG He-qing |
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| Institution: | FANG Xue-ping,HUANG He-qing~* |
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| Abstract: | In this article, a novel method of SDS-PAGE is introduced for analyzing proteins and subunits with MALDI-TOF mass spectrometry. We used copper stain to visualize the proteins and subunits in SDS polyacrylamide gels. The subunits were extracted from gel by using organic solution. Then we analyzed their molecular weight with the technology of mass spectrometry directly without further disposal. Moreover, this method has advantages of simple, fast and low loss rate. And it is especially suitable for the study of proteomics, analysis of protein homologue with database and kinds of confirming proteins etc. It is suggested that the method of coupling both SDS-PAGE and MALDI-TOF mass spectrometry be a perfect analyzing technology in the off-line. |
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| Keywords: | copper stain SDS-PAGE MALDI-TOF subunits molecular weight |
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