| 基于DPPH-HPLC技术的马粪海胆抗氧化活性指纹图谱初步研究 |
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| 引用本文： | 赵恒强,陈军辉,耿岩玲,刘建华,王晓.基于DPPH-HPLC技术的马粪海胆抗氧化活性指纹图谱初步研究[J].山东科学,2014,27(3):7-11. |
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| 作者姓名： | 赵恒强 陈军辉 耿岩玲 刘建华 王晓 |
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| 作者单位： | 1.山东省科学院中药过程控制研究中心，山东省大型精密分析仪器应用技术重点实验室，山东省分析测试中心，;
山东 济南 250014；
2. 国家海洋局第一海洋研究所，青岛市现代分析技术及中药标准化重点实验室，;
山东 青岛266061 |
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| 基金项目： | 山东省自然科学基金(ZR2013HL029);山东省科学院科技发展基金(2012015);济南市科技明星计划(2013030) |
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| 摘 要： | 采用基于DPPH-HPLC技术的抗氧化活性指纹图谱对海洋中药马粪海胆来源进行鉴别。对具有较好抗氧化活性的马粪海胆水提物中的抗氧化活性成分进行筛选,基于各抗氧化成分的指纹图谱,结合相似度分析对不同来源马粪海胆进行鉴别研究。结果表明,该方法可以对不同来源的马粪海胆样品进行正确区分,有望成为海洋中药真伪鉴别的新方法。
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| 关 键 词： | 马粪海胆 DPPH-HPLC 抗氧化 指纹图谱 |
| 收稿时间： | 2014-01-15 |
DPPH-HPLC based preliminary research on antioxidant activity fingerprint for Hemicentrotus pulcherrimus(A. Agassiz) |
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| ZHAO Heng-Qiang,CHEN Jun-Hui,GENG Yan-Ling,LIU Jian-Hua,WANG Xiao.DPPH-HPLC based preliminary research on antioxidant activity fingerprint for Hemicentrotus pulcherrimus(A. Agassiz)[J].Shandong Science,2014,27(3):7-11. |
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| Authors: | ZHAO Heng-Qiang CHEN Jun-Hui GENG Yan-Ling LIU Jian-Hua WANG Xiao |
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| Institution: | 1. Shandong Provincial Key Laboratory of Applied Technology of Sophisticated Analytical Instruments,Process Control Research Center for Traditional Chinese Medicine, Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014,China;
2. Qingdao Key Labortory of Analytical Technology Development and Standardization of Chinese Medicines,;
The First Institute of Oceanography, SOA, Qingdao 266061, China |
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| Abstract: | We identify the origin of Hemicentrotus pulcherrimus(A. Agassiz) with a DPPH-HPLC based antioxidant fingerprint. DPPH-HPLC was employed to screen the antioxidant of water extract of Hemicentrotus pulcherrimus.Antioxidant based fingerprint combined with similarity analysis was employed to identify the authenticity of different Hemicentrotus pulcherrimus. Results show that the approach can distinguish Hemicentrotus pulcherrimus from different origins. Thus, it has the potential to become a new method for the identification of marine Chinese medicines. |
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| Keywords: | Hemicentrotus pulcherrimus(A Agassiz) DPPH-HPLC antioxidant fingerprint |
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