| 上海四膜虫酚溶性染色质非组蛋白的研究 |
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| 引用本文： | 沈锡祺,候连生.上海四膜虫酚溶性染色质非组蛋白的研究[J].华东师范大学学报(自然科学版),1997(4):87-90. |
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| 作者姓名： | 沈锡祺 候连生 |
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| 作者单位： | 华东师范大学生物系!上海，200062，华东师范大学生物系!上海，200062，华东师范大学生物系!上海，200062 |
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| 摘 要： | 收集对数生长期的上海四膜虫，分离细胞核，制备染色质并以酚抽提制得酚溶性染以质非组蛋白，经ＳＤＳ－ＰＡＧＥ测得四膜虫酚溶性染色非组蛋白有六条明显的蛋白带，其表观量为８８ＫＤ，７９ＫＤ，７０ＫＤ，５２ＫＤ，３８ＫＤ，２４ＫＤ，与鼠肝酚溶性染色质非组蛋白具有明显的５条的分子量有显著的差异。
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| 关 键 词： | 原生动物 上海四膜虫 酚溶性 染色质 非组蛋白 |
Study of Chromatin's Non-Histones Dissolved in Phenol from Tetrahymena shanghaiensis SI |
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| Shen Xiqi, Hou Lianshen, Zhu Wei.Study of Chromatin''''s Non-Histones Dissolved in Phenol from Tetrahymena shanghaiensis SI[J].Journal of East China Normal University(Natural Science),1997(4):87-90. |
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| Authors: | Shen Xiqi Hou Lianshen Zhu Wei |
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| Abstract: | The paper describes T. shanghaiensis SI collected in logarithmic growth phase, in which the nuclei is isolated, the chromatin is prepared and the chromatin's non - histones are extracted in phenol. Then analyzed by SDS - PAGE. The results indicate: The non - histones dissolved in phenol. from T. shanghaiensis SI have six protein bands and their apparent M.W. are 88KD, 79KD, 70KD, 52KD, 38KD and 24KD respectively, these are very different as compared with rat lever cells. |
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| Keywords: | protozoa T shanghaiensis non-histones SDS-PAGE |
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