离子注入对三角叶滨藜种子萌发及愈伤组织的影响

利用N^ 、Ar^ 、Zn^2 离子分不同辐射能量，对三角叶滨藜（Atriplex Triangularis）种子进行离子注入，研究低能量状态下激发诱变的生物学效应。结果表明N^ 、Ar^ 、Zn^2 离子注入处理后，三角叶滨藜种子萌发率均有下降，其中Ar^ 处理组种子萌发率高于对照组。由于菌苗诱导产生愈伤组织及其生理活性也存在差异。通过对愈伤组织SOD、POD、CAT活性进行测定，发现这3种离子注入种子处理后愈伤组织的SOD、POD、CAT活性一般都低于未处理的对照组。其中Zn^2 注入处理后SOD活性高于对照组。

Effects of N~+ Ar~+ Zn~(2+) Ion Implanation on Seed Germination and Calli Growth of Atriplex Triangularis

Atriplex triangularis , a kind of halophyte species, was used as experimental material. Effects of growth of their calli and activities of their superoxide dismutase(SOD) catalase(CAT)and peroxidase(POD)were investigated. The results showed that the rate of seed germination by N+ , Ar+ , Zn2+ implantation were different from that of control. The rate of seed germination were 28. 6% , 34. 8% and 11.1% when the energy of N+ implanted were 30,45 and 60 kV, respectively. The higher the energy of Zn2+ treated showed, the lower the rate of seed germination would be. Effects of the dif- ferent treatment energy of Ar+ on the rate of seed germination were not obvious. After the treatment of N+ , Ar+ , Zn2+ ,the seeds of Atriplex triangularis were induced into sterile seedlings,then the ster- ile seedlings were induced into calli,and activities of SOD,POD and CAT in calli took apparent chan- ges. The activities of SOD,POD and CAT by N+ ,Ar+ treatment were lower than those of control;the activity of SOD by Zn2+ treatment was much higher than those of control. Moreover, the sterile seed- lings by Zn2+ treatment grow vigorously,the growth of their calli was comparatively slow.