| 神经营养因子NT-3神经干细胞移植对帕金森病大鼠功能修复研究 |
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| 引用本文： | 毕建清,姚远,文铁桥.神经营养因子NT-3神经干细胞移植对帕金森病大鼠功能修复研究[J].科技导报(北京),2009,27(14):48-53. |
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| 作者姓名： | 毕建清 姚远 文铁桥 |
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| 作者单位： | 毕建清,姚远,BI Jianqing,YAO Yuan(上海大学生命科学学院,上海,200444);文铁桥,WEN Tieqiao(上海大学生命科学学院,上海,200444;上海大学系统生物技术研究所,上海,200444) |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 为了研究神经干细胞和神经营养因子NT-3对帕金森病(PD)的修复作用,将NT-3表达载体pEGFP-C1-NT3转染大鼠原代神经干细胞(NSCs),构建了NSCs-NT3细胞.移植入帕金森病模型大鼠脑部MFB和VTA区域,观察到移植细胞在大鼠移植部位生长与迁移,证实移植成功.对移植后疾病模型进行APO旋转实验和水迷宫行为检测,结果表明,NT-3有利于学习与记忆能力改善,分子水平证实移植区域NT-3显著表达.本研究通过移植过表达NT-3的神经干细胞,从分子、细胞、组织和个体行为等不同层面证实了NSCs-NT3对PD模型大鼠修复的积极作用,为PD的转基因神经干细胞移植治疗研究提供了理论与实践依据.
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| 关 键 词： | 帕金森病 神经干细胞 神经营养因子-3 移植 |
Effects of Neurotrophic Factor NT-3 and Neural Stem Cell on the Function Repair for Parkinson Disease Model |
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| BI Jianqing,YAO Yuan,WEN Tieqiao, .School of Life Sciences,Shanghai University,Shanghai ,China.Effects of Neurotrophic Factor NT-3 and Neural Stem Cell on the Function Repair for Parkinson Disease Model[J].Science & Technology Review,2009,27(14):48-53. |
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| Authors: | BI Jianqing YAO Yuan WEN Tieqiao School of Life Sciences Shanghai University Shanghai China |
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| Institution: | BI Jianqing1,YAO Yuan1,WEN Tieqiao1,2 1.School of Life Sciences,Shanghai University,Shanghai 200444,China 2.Institute of Systems Biology,China |
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| Abstract: | Neural stem cell transplantation is one of the important ways for repair of nerve injury.In this study,the effects of neural stem cells and neurotrophic factor-3(NSCs-NT3) on the Parkinson disease model are studied.By transplantation of NSCs-NT3 into lesion regions of PD rats,rotational behavior and Morris Water Maze(MWM) were tested.The results show that the rotation numbers in apomorphine-induced NSCs-NT3 group showed a higher decrease than that of NSCs group.MWM training also suggested NSCs-NT3 improved ... |
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| Keywords: | Parkinson's disease neural stem cells NT-3 transplantation |
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