| 手性固定相合成中原料YWG-NH_2与Spher-NH_2性能的比较 |
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| 引用本文： | 杨国生,戴庆,高如瑜,沈含熙,陈茹玉,王琴荪,李庆英.手性固定相合成中原料YWG-NH_2与Spher-NH_2性能的比较[J].山东大学学报(理学版),1997(2). |
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| 作者姓名： | 杨国生 戴庆 高如瑜 沈含熙 陈茹玉 王琴荪 李庆英 |
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| 作者单位： | 山东大学化学系，南开大学化学学院，济南聚大纤维有限公司 |
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| 摘 要： | 利用液相色谱手性固定相进行手性分离的研究越来越多，因此手性固定相的合成也就成了该项研究中必不可少的关键问题．在手性固定相的合成中氨丙基硅胶作为手性固定相的键合基体，其性质的好坏直接影响手性固定相的手性分离能力．分别利用国产和进口分装的氨丙基硅胶作基体，合成结构完全相同的手性固定相，结果表明进口的氨丙基硅胶合成的手性固定相的性能优于用国产氨丙基硅胶合成的手性固定相，并论述了可能产生这种差别的原因．
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| 关 键 词： | 手性固定相 氨丙基硅胶 手性固定相合成 |
STUDIES ON THE PROCESS OF SOMATIC EMBRYOGENESIS OF GRAPEVINE IN VITRO CULTURE I. INVESTIGATION ON HISTOLOGICAL CYTOLOGY OF THE SOMATIC EMBRYOGENESIS |
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| Hou,Bingkai,Bao,Xuezhen,Yu,Huimin,Wang,Minqin,Zhuo,Deshuo.STUDIES ON THE PROCESS OF SOMATIC EMBRYOGENESIS OF GRAPEVINE IN VITRO CULTURE I. INVESTIGATION ON HISTOLOGICAL CYTOLOGY OF THE SOMATIC EMBRYOGENESIS[J].Journal of Shandong University,1997(2). |
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| Authors: | Hou Bingkai Bao Xuezhen Yu Huimin Wang Minqin Zhuo Deshuo |
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| Abstract: | Anthers and filaments of grapevine (vitis vinifera L.)were used for the investigation on histological cytology of the somatic embryogenesis.The results of investigation showed that(1) embryogenic and nonembryogenic callus were formed after explants were incubated onto inducing medium.Somatic embryos were formed indirectly from the embryogenic callus. (2) Somatic embryogenesis was initiated from the single embryogenic cell which lied on the epidermal or subepidermal layers of callus,and had bigger nuclei,thicker cytoplasm and denser colour.The single embryogenic cell got isolated from each other,then they began to Mitosis.The first time was equal or unequal,more division were carried out and the two cell,three cell,and multicellular proembryos were formed.The proembryo continued to develop into the cotyledonary embryo. (3) The development of somatic embryos of grapevine was similar to that of the zygotic embryos in dicotyledon. |
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| Keywords: | grapevine(Vitis vinifera L ) somatic embryo histological cytology |
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