| 超氧化物歧化酶的异硫氰酸荧光素脂质体的研究 |
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| 引用本文： | 潘继承,汪劲松,黄阜峰,邱昌恩,钟兵.超氧化物歧化酶的异硫氰酸荧光素脂质体的研究[J].湖北师范学院学报(自然科学版),1997(3). |
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| 作者姓名： | 潘继承 汪劲松 黄阜峰 邱昌恩 钟兵 |
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| 作者单位： | 湖北师范学院生物系 |
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| 基金项目： | 湖北省教委重点资助项目 |
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| 摘 要： | 在pH9.5的0.5mol/LNa_2CO_3缓冲液中,异硫氰酸荧光素(FITC)标记牛血超氧化物歧化酶(Cu,Zn-SOD),经层析得到纯化的FITC-SOD;用逆相蒸发法制得含FITC-SOD的逆相蒸发囊泡(REV)即(FITC-SOD)-LUV,将含(FITC-SOD)-LUV与游离的FITC-SOD的混合物,通过—Sepharose4B层析柱,合并洗脱后的A_(620)峰即为(FITC-SOD)-LUV.
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| 关 键 词： | 牛血Cu Zn-SOD 异硫氰酸荧光素 脂质体 |
STUDY OF FLUORESCEIN ISOTHIOCYNATE' S LIPOSOMES CONTAINING SUPEROXIDE DISMUTATE |
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| Pan Jicheng Wang Jinsong Huang Fufeng Qiu Changen.STUDY OF FLUORESCEIN ISOTHIOCYNATE'''' S LIPOSOMES CONTAINING SUPEROXIDE DISMUTATE[J].Journal of Hubei Normal University(Natural Science),1997(3). |
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| Authors: | Pan Jicheng Wang Jinsong Huang Fufeng Qiu Changen |
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| Institution: | Department of Biolagy |
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| Abstract: | In this paper,Bovine superoxide dismutate (SOD)was labelled with Fluorescein isothiocynate(FITC). Large unilamellar vesicles(LUV) containing FITC-SOD was gained by Reverse Evaporation,and it was purifed by Sepharose 4B chromatography. |
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| Keywords: | Bovine superoxide dismutate Fluorescein isothiocynate Liposome |
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