| 不同环境中胞外多糖产生菌的初步研究(英文) |
| |
| 引用本文： | 马晶璟,尹若春.不同环境中胞外多糖产生菌的初步研究(英文)[J].安徽大学学报(自然科学版),2011(3). |
| |
| 作者姓名： | 马晶璟 尹若春 |
| |
| 作者单位： | 安徽大学生命科学学院; |
| |
| 基金项目： | University Outstanding Teacher's Foundation of Anhui Province(2004JQ114); Natural Science Foundation of Anhui Provincial Education Department (2004KJ022); Natural Science Foundation of Anhui Province(090413083) |
| |
| 摘 要： | 从不同的环境中采集样品,采用LB平板稀释法和苯胺蓝法分析样品中胞外多糖产生菌的丰度并分离胞外多糖产生菌;以发酵产糖量分析进行复筛,对产糖量较高的菌株进行常规生理生化分析和16SrDNA序列分析.结果表明:不同环境中胞外多糖产生菌的数量和类别有较大差异,其中活性污泥中胞外多糖产生菌数量最多;复筛得到89个菌株,选择其中8个产糖量较高的菌株进行生理生化实验和16SrDNA序列分析,结果显示:4个菌株属于芽孢杆菌属,另外4个可能属于新的细菌菌株.
|
| 关 键 词： | 胞外多糖 细菌 16SrDNA |
Primary study on extracellular polysaccharide producing bacteria in different environments |
| |
| MA Jing-jing,YIN Ruo-chun.Primary study on extracellular polysaccharide producing bacteria in different environments[J].Journal of Anhui University(Natural Sciences),2011(3). |
| |
| Authors: | MA Jing-jing YIN Ruo-chun |
| |
| Institution: | MA Jing-jing,YIN Ruo-chun (School of Life Science,Anhui University,Hefei 230039,China) |
| |
| Abstract: | To isolate extracellular polysaccharides(EPS) producing bacteria,samples from different environments were collected and analyzed with LB dilution stained by Aniline Blue.Results showed that the number and diversity of extracellular polysaccharides producing bacteria from different environments were variable.89 strains were obtained to produce yield of EPS.8 strains which gave higher yield were selected to carry on physiological and biochemical tests and 16SrDNA sequence analysis.The results indicate that 4 ... |
| |
| Keywords: | extracellular polysaccharide bacteria 16SrDNA |
| 本文献已被 CNKI 等数据库收录！ |
