| 荷花胚组织培养的初步研究 |
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| 引用本文： | 孔德政,李艳妮,杨秋生,刘广甫.荷花胚组织培养的初步研究[J].河南科学,2007,25(4):593-595. |
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| 作者姓名： | 孔德政 李艳妮 杨秋生 刘广甫 |
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| 作者单位： | 1. 河南农业大学,林学园艺学院,郑州,450002
2. 郑州市第二苗圃,郑州,450052 |
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| 基金项目： | 河南省杰出青年科学基金 |
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| 摘 要： | 以荷花胚为试材,研究不同生长调节剂、基本培养基、外植体消毒时间对离体荷花胚萌发的影响.结果表明,诱导胚萌发较理想的培养基为MS+6-BA0.5 mg/L+2,4-D0.2 mg/L+0.1%AC+3%蔗糖;MS+6-BA1.0 mg/L+NAA0.5 mg/L+0.1%AC+3%蔗糖,光照14h.d-1.总结了荷花胚组织培养上存在的问题,并对解决某些问题提出了一些建议.
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| 关 键 词： | 荷花 胚 组织培养 萌发 |
| 文章编号： | 1004-3918（2007）04-0593-03 |
| 修稿时间： | 2007-03-12 |
The Preliminary Research on Tissue Culture of Lotus |
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| KONG De-zheng,LI Yan-ni,YANG Qiu-sheng,LIU Guang-pu.The Preliminary Research on Tissue Culture of Lotus[J].Henan Science,2007,25(4):593-595. |
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| Authors: | KONG De-zheng LI Yan-ni YANG Qiu-sheng LIU Guang-pu |
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| Institution: | College of Forestry and Horticulture , Henan Agricultural University, Zhengzhou 450002, China ;2. The Second Nursery in Zhengzhou City, Zhengzhou 450052, China |
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| Abstract: | Factors which affect germination embryos of Nelumbo nucifer Lotus were studied.Results showed that the optimum medium for embryo culture were MS+6-BA0.5 mg/L +2,4-D0.2 mg/L + 0.1 %AC+3 % sucrose and MS+6-BA1.0 mg/L +NAA0.5 mg/L+ 0.1 % AC +3 % sucrose,light were 14 h·d-1.And summarized the questions which the lotus embryo tissue culture existing and proposed to the suggestions of some questions.These are very significant to enhance the reproduction coefficient of the the lotus. |
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| Keywords: | lotus embryo tissue culture germination |
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