| 普通级啮齿类实验动物部分细菌携带情况的初步调查 |
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| 引用本文： | 范薇,隋丽华,刘永梅,田克恭,贺争鸣.普通级啮齿类实验动物部分细菌携带情况的初步调查[J].实验动物科学,2001,18(2):18-20. |
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| 作者姓名： | 范薇 隋丽华 刘永梅 田克恭 贺争鸣 |
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| 作者单位： | 1. 军事医学科学院实验动物中心,北京,100071
2. 中国药品生物制品检定所,北京,100050 |
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| 基金项目： | 科技部科研课题"清洁级实验小鼠微生物和遗传检测试剂盒的研究"部分内容(TJ98-SJ03). |
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| 摘 要： | 了解在实际生产中常用普通级啮齿类实验动物中清洁级规定排除的4种主要细菌(沙门菌、鼠棒状杆菌、嗜肺巴斯德杆菌、支气管败血性鲍特菌)的携带状况,以及验证筛选的实验动物细菌检测常用普通培养基和生化培养基的应用效果.方法无菌采集动物气管分泌物,接种血平皿;采集回盲部内容物接种DHL、SS琼脂平皿,37℃48h后挑取可疑菌落进行纯化,取纯培养物进行染色镜检、生化特性鉴定及血清学反应.结论KM小鼠嗜肺巴斯德杆菌阳性率最高,为60%;豚鼠携带支气管败血性鲍特菌,阳性率为30%;总的细菌检出率为20%(其中嗜肺巴斯德杆菌总阳性率为16.25%,支气管败血性鲍特菌总阳性率为3.75%);选出的培养基应用于实验检测中是可行的.
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| 关 键 词： | 啮齿类实验动物 细菌 携带 |
| 文章编号： | 1006-6179(2001)02-0018-03 |
| 修稿时间： | 2000年10月10 |
Investigating Bacterial Infection in Conventional Laboratory Rodents |
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| FAN Wei,Sui Li-hua,LIU Yong-mei,TIAN Ke-gong,HE Zheng-ming.Investigating Bacterial Infection in Conventional Laboratory Rodents[J].Shiyan Dongwu Kexue,2001,18(2):18-20. |
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| Authors: | FAN Wei Sui Li-hua LIU Yong-mei TIAN Ke-gong HE Zheng-ming |
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| Institution: | Laboratory Animal Centre of Academy of Militrary Medical Sciences Beijing\ 100071 1.National Institute for the Control of Pharmaceutical and Biological Products Beijing\ 100050 |
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| Abstract: | For seeing partial bacteria of cleaning animals in conventional laboratory rodents and validating the effect of the culture media selected,8 strains of laboratory rodent were detected.The results showed that the infective rates of Pasteurella Pneumotropica in KM mice were 60%,C\-\{57\} mice were 10%,SD rats were 10%,Wistar rats were 20%; Bordetella Bronchiseptica in Guinea pigs were 30%.Total rates of bacterial infection were 20% (Pasteurella Pneumotropica were 16.25 %,Bordetella Bronchiseptica were 3.75%).The selected culture media was effective. |
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| Keywords: | laboratory rodents bacteria infection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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