| 瓢虫干标本基因组DNA的提取及RAPD分析 |
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| 引用本文： | 张迎春,郑哲民.瓢虫干标本基因组DNA的提取及RAPD分析[J].西北大学学报,1999,29(6):581-583. |
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| 作者姓名： | 张迎春 郑哲民 |
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| 作者单位： | 陕西师范大学生命科学学院!陕西西安710062 |
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| 基金项目： | 陕西省自然科学基金!No．97SM10 |
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| 摘 要： | 实验对保存多年的瓢虫干标本进行了基因组 ＤＮＡ提取和 ＲＡＰＤ－ＰＣＲ扩增。结果显示 ＤＮＡ分子的提取与保存年代长短无直接关系；ＲＡＰＤ－ＰＣＲ增产物经琼脂糖凝胶电泳检测，分子量大小约为 １９８４ｂｐ～６３０ｂｐ；不同种瓢虫的 ＤＮＡ用同一引物，扩增片段是多态表现，同一种瓢虫的干标本保存时间虽不同，但扩增产物中均具有相同的ＤＮＡ片段。
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| 关 键 词： | 瓢虫 干标本 RAPD-PCR DNA |
RAPD analysis of genomic DNA specimens of dry coccinellid specimens |
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| ZHANG Ying-chun,ZHENG Zhe-min.RAPD analysis of genomic DNA specimens of dry coccinellid specimens[J].Journal of Northwest University(Natural Science Edition),1999,29(6):581-583. |
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| Authors: | ZHANG Ying-chun ZHENG Zhe-min |
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| Abstract: | The Genomic DNA was extracted and amplified from dry samples of coccinellids (Coleoptera coccinellidae). Whether the DNA could be extracted has no relationship with preserving time. The DNA fragments amplified with RAPD-PCR were examined by agarose gel electrophoresis (AGE). Its molecular weight was 1 984 hp to 360 hp. DNAs of different species amplified by same primer show polymorphism. Although dry samples of same species were different preserving time,the DNA amplified from same species has same fragments. |
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| Keywords: | coccinellids genomic DNA RAPD anoalysis |
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