Circular dichroism spectroscopic studies on structures formed by telomeric DNA sequencesin vitro |
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| Authors: | Xiaoyan Zhang Enhua Cao Xueguang Sun Chunli Bai |
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| Institution: | (1) Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Institute of Chemistry, Chinese Academy of Sciences, 100101 Beijing, China |
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| Abstract: | Telomere plays an important role in cellular processes, such as cell aging, death and carcinogenisis. Having special sequences,
it can form quadruplex structurein vitro. Circular dichroism (CD) spectroscopic studies show that TTAGGG, (TTAGGG)2 and (TTAGGG)4 can all form quadruplexin vitro and exist mainly as parallel quadruplex without metal ions. Both K+ and Na+ can stabilize the tetrameric structure and facilitate the forming of anti-parallel conformation. Furthermore, the conformations
of quadruplex can also be affected by sequence length, the nature and concentration of metal ions. |
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| Keywords: | human telomere quadruplex conformation CD spectrum K+ Na+ cation concentration |
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