| 马蹄莲组培过程中真菌和细菌污染的消除方法研究 |
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| 引用本文： | 李群,陈丽萍,石轶松.马蹄莲组培过程中真菌和细菌污染的消除方法研究[J].四川师范大学学报(自然科学版),2001,24(6):607-609. |
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| 作者姓名： | 李群 陈丽萍 石轶松 |
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| 作者单位： | 1. 四川师范大学化学与生命科学学院
2. 重庆大学生物技术研究所 |
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| 摘 要： | 以生长于土壤中易污染的马蹄莲小块茎为试材，对其降低污染措施进行研究：接种前先用杀菌剂（代森锰锌）对外植体进行预处理，后用不同种类、不同浓度的抗菌素处理，结果表明：用浓度为3g/L的杀菌剂，进行24h处理使污染率下降了52.97%，接着采用正交实验，用200mg/L氨苄西林钠、250mg/L硫酸链霉素、6.25mg/L硫酸庆大霉素，进行24h处理使污染率下降到21.34%，效果十分明显。
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| 关 键 词： | 马蹄莲 组织培养 污染 消除 真菌 细菌 |
| 文章编号： | 1001-8395(2001)06-0607-03 |
| 修稿时间： | 2001年4月10日 |
Study on Methods of Elimimation of Fungi and Bacteria Contamination in Tissue Culture of Zantedeschia |
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| LI Qun ,CHEN Li-ping ,SHI Yi-song.Study on Methods of Elimimation of Fungi and Bacteria Contamination in Tissue Culture of Zantedeschia[J].Journal of Sichuan Normal University(Natural Science),2001,24(6):607-609. |
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| Authors: | LI Qun CHEN Li-ping SHI Yi-song |
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| Institution: | LI Qun 1,CHEN Li-ping 1,SHI Yi-song 2 |
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| Abstract: | We study a method of elimimation of fungi and bacteria contamination in Zantedeschia aethipica rhizomes. The rhizomes are pretreated first by germicide (Dithane M-45) and then treated by different antibiotic treatments. The results indicate that contamination percent decreases to 52.97% by 24 hours treatment with 3 g/L germicide, and to 21.34% by 24 hours one with 200 mg/L Ampicillin 250 mg/L Streptomycin 6.25 mg/L. This method is effective. |
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| Keywords: | Zantedschia aethiopica Spreng Tissue culture Contamination Elimimation |
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