| 氨基化的介孔二氧化硅纳米材料与pDNA作用 |
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| 引用本文： | 郑昆,杨红,葛雅琨,隋新,温刚,张元新.氨基化的介孔二氧化硅纳米材料与pDNA作用[J].吉林大学学报(理学版),2015,53(6):1316-1320. |
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| 作者姓名： | 郑昆 杨红 葛雅琨 隋新 温刚 张元新 |
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| 作者单位： | 1. 吉林化工学院 生物与食品工程学院, 吉林 吉林 132022; 2. 吉林工贸学校, 吉林 吉林 132021 |
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| 摘 要： | 采用共沉淀法合成高度有序、分散性良好的氨基化介孔二氧化硅纳米材料(Am-MSNs),并研究其吸附和保护质粒DNA(pDNA,PEGFP-N3)的性质,测定样品材料对A549和HeLa细胞的生物相容性.实验结果表明:Am-MSNs能有效吸附PEGFP-N3,并保护PEGFP-N3免受限制性内切酶的消化;Am-MSNs对A549和HeLa细胞表现出较高的生物相容性,当材料的质量浓度为125μg/mL时,两种细胞的存活率均约为100%.
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| 关 键 词： | 氨基化介孔二氧化硅纳米材料 质粒DNA 吸附 保护 生物相容性 |
| 收稿时间： | 2015-04-29 |
Amino-functionalized Mesoporous Silica Nanoparticles Interacting with pDNA |
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| ZHENG Kun,YANG Hong,GE Yakun,SUI Xin,WEN Gang,ZHANG Yuanxin.Amino-functionalized Mesoporous Silica Nanoparticles Interacting with pDNA[J].Journal of Jilin University: Sci Ed,2015,53(6):1316-1320. |
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| Authors: | ZHENG Kun YANG Hong GE Yakun SUI Xin WEN Gang ZHANG Yuanxin |
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| Institution: | 1. College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, Jilin Province, China; 2. Jilin Industry and Trade School, Jilin 132021, Jilin Province, China |
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| Abstract: | This work evaluated the adsorption and protection capacities of amino functionalized mesoporous silica nanoparticles (Am-MSNs) for pDNA （PEGFP-N3）. The in vitro biocompatibilities of Am MSNs to A549 and Hela cells were also tested, respectively. The results reveal that the Am-MSNs
exhibit good adsorption capacity for PEGFP N3. Moreover, the material can also protect PEGFP N3 from enzymatic degradation completely. In addition, the
sample presents high in vitro biocompatibilities to HeLa and A549 cells. |
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| Keywords: | amino-functionalized mesoporous silica nanoparticle(Am-MSNs) pDNA adsorption protection biocompatibility |
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