体外培养人涎腺多形性腺瘤细胞及表面标记物的初步研究

体外培养15例人涎腺多形性腺瘤原发肿瘤组织，探讨体外培养的细胞与原发肿瘤组织的表面标志物表达情况。运用免疫组化法检测CK5/6、CK7、CK8/18、CK14、CK20，vimentin及肌上皮表面标记物α-SMA、calponin及p63在细胞和肿瘤组织中的表达。结果发现体外培养的多形性腺瘤细胞呈多样形，可同时表达角蛋白、肌上皮表面标记物及波形蛋白，与原发肿瘤表达基本一致。提示体外培养的多形性腺瘤细胞可能大部分来源于肌上皮细胞及导管上皮细胞等，这为研究涎腺多形性腺瘤肿瘤生物学多样性提供了实验依据。

Characteristics of human salivary pleomorphic adenoma cells cultured in vitro and the surface markers

To study the biological characteristics of salivary gland pleomorphic adenoma tumor.Fifteen tumor tissue derived from salivary gland pleomorphic adenoma were cultured in vitro.Immunohistochemical staining was used to investigate the expression of surface markers such as CK5/6,CK7,CK8/18,CK14,CK20,p63,α-SMA,calponin,vimentin.The results showed that the cultured human salivary pleomorphic adenoma cells were varied and expressed both keratin and myoepithelial cell surface markers which indicated that the cells may be from myoepithelial cells and ductal epithelial cells.The results provide experimental basis for the study of the multiple biological characteristics of salivary gland pleomorphic adenoma tumor.