| SDS-PAGE电泳法测定蒜头果蛋白的相对分子质量 |
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| 引用本文： | 袁燕,王红斌,BARROW Colin J,杨文荣.SDS-PAGE电泳法测定蒜头果蛋白的相对分子质量[J].云南民族大学学报(自然科学版),2015(2):123-125. |
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| 作者姓名： | 袁燕 王红斌 BARROW Colin J 杨文荣 |
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| 作者单位： | 云南民族大学民族药资源化学国家民委-教育部重点实验室;云南民族大学云南省生物高分子功能材料工程技术研究中心;澳大利亚迪肯大学生命与环境科学学院化学与生物技术中心 |
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| 基金项目： | 国家自然科学基金(81260500);教育国际化平台建设项目(218-02001001002129);云南省“无机化学精品课程”资助项目(2012HX08,2012HX12) |
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| 摘 要： | 蒜头果蛋白(malanin)是从广西、云南特有植物蒜头果中分离、纯化出的一种新的、具有抗肿瘤活性的植物蛋白质.采用变性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定malanin的相对分子质量.结果表明,malanin是一种双链蛋白质,由A、B 2条肽链通过二硫键连接而成,其相对分子质量约为65.94×103.方法具有简单、快速、直观的特点.
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| 关 键 词： | 蒜头果蛋白 SDS-PAGE 相对分子质量 |
Determination of the relative molecular mass of malanin with SDS-PAGE |
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| YUAN Yan;WANG Hong-bin;BARROW Colin,J;YANG Wen-rong.Determination of the relative molecular mass of malanin with SDS-PAGE[J].Journal of Yunnan Nationalities University:Natural Sciences Edition,2015(2):123-125. |
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| Authors: | YUAN Yan;WANG Hong-bin;BARROW Colin J;YANG Wen-rong |
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| Institution: | ;Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission and Ministry of Education of China,Yunnan Minzu University;Engineering Research Center of Biopolymer Functional Materials of Yunnan,Yunnan Minzu University;Center for Chemistry and Biotechnology,School of Life and Environmental Sciences,Deakin University; |
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| Abstract: | Malanin,a novel plant protein with anti-tumor activities,was isolated and purified from an endemic plant of malania oleifera in Guangxi and Yunnan provinces. The relative molecular mass of malanin was determined by the denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis( SDS-PAGE). The result has showed that malanin is a protein with two chains,chain-A and chain-B,which are cross-linked by one or two disulfide bonds,and its relative molecular mass is about 65 94 × 103. SDS-PAGE gel electrophoresis is a simple,fast,direct-viewing method to determine the relative molecular mass of malanin. |
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| Keywords: | malanin SDS-PAGE relative molecular mass weight |
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