豇豆种子萌发进程中蛋白质组分的时空变化

以豇豆品种(早翠和矮虎)为材料,通过SDS-PAGE技术获得3个时期(干种子期、吸胀期、发芽期)和2个部位胚(胚芽、胚根和胚轴)和子叶]的3种蛋白质组分(水溶、盐溶和碱溶)的电泳图谱.结果表明:品种间条带分布差异最大的时期是干种子期,部位是胚,组分为盐溶蛋白质.在萌发进程中,相对分子质量为60.4KD、55.4KD和49.7KD的3个条带没有时空变化,推测其是豇豆的保守性蛋白或结构蛋白.组分中以水溶蛋白所显条带数居多,但品种间多态性不丰富,早翠有两个特异带,而矮虎仅一个;盐溶蛋白组分所显品种间条带多态性丰富,早翠有107.8KD、18.3KD、15.7KD、14.0KD、12.8KD和11.4KD6个特异带,矮虎有41.0KD、27.3KD和14.4KD3个.故在豇豆种子萌发进程中,各蛋白质组分在品种内和品种间的时序和空间表达上,既显示出一致性又显示出差异性.由于对干种子期胚或子叶盐溶蛋白质组分的电泳分析,既显示品种遗传稳定性又能反映品种特异性,故均适宜于豇豆基因型判别,但基因型遗传关系分析宜以胚为材料,品种纯度检测宜用子叶为材料,不能两者混合取材,吸胀后的种子蛋白质组分分析不能反映基因型差异.

Changes of Protein Components in Different Positions and Stages of Germination in Yardlong Bean (Vigna sesquipedalis (L.) Fruhw.)

Changes of protein components in different positions and periodsof seed germination in yardlong bean were studied by SDS-PAGEtechnique.The electrophoresis patternsof threeproteincomponents,i.e.water soluble proteins,salt soluble proteins(vignin) and alkali soluble proteinsin two positions(embryo and cotyledon) at three stages(dry seed,swollen seed and burgeonseed) of Vigna sesquipedalis cultivars ‘Zaocui’ and ‘Aihu’ were gained.The results showed that the number of subunits as well as their loci in embryo of dry seed derived from vignin profiles was affluent among cultivars.Several subunits such as 60.4KD,55.4KD and 49.7KD in molecularweight(MW) were found at all stages in all components either from embryo or cotyledon.They were guessed to be expressions of conservative genes or to be structural proteins in yardlong bean.In water soluble protein profile,only two distinct subunits with MW of 27.3KD and 24.9KD in ‘Zaocui’ and oneof 10.2KDin‘Aihu’ were foundthoughtheir totalnumber of subunits was the biggest among the three components.The profiles of vignin showed polymorphism,the distinct subunits of 107.8KD,18.3KD,15.7KD,14.0KD,12.8KD and 11.4KD were denoted in‘Zaocui,’ so were 41.0KD,27.3KD and 14.4KD in ‘Aihu.’ Hence the number and the locus of subunitsde-rived from three components varied from each other as well as identical.It suggested that the spa-tiotemporal coherence and otherness be existed on the expressions of genes between and within cul-tivars.The profiles derived from proteins in embryo and cotyledon varied each other regardless of germination stages and protein components.Therefore,it was available to identify the genetic di-versity by exam the profile of vignin of dry seed embryo or cotyledon from yardlong bean cultivars.