| 中国红豆杉悬浮细胞培养动力学的研究 |
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| 引用本文： | 王红强,钟建江.中国红豆杉悬浮细胞培养动力学的研究[J].华东理工大学学报(自然科学版),1997,23(6):679-683. |
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| 作者姓名： | 王红强 钟建江 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室 |
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| 摘 要： | 考察了中国红豆杉悬浮细胞培养中细胞生长的动态变化，培养基中主要营养成分的消耗，细胞的氧消耗速率，以及次级代谢产物紫杉醇的生产状况。实验结果表明，细胞比生长速率为０．１２９／ｄ，紫杉醇的积累与细胞生长呈部分相关在，培养静止期达到高峰。
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| 关 键 词： | 细胞培养 动力学 中国 红豆杉 紫杉醇 |
Kinetic Study on Suspension Cultures of Taxus Chinensis for Production of Anticancer Drug Taxol |
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| Wang Hongqiang,Zhong Jianjiang ,Chen Xufeng and Yu Juntang.Kinetic Study on Suspension Cultures of Taxus Chinensis for Production of Anticancer Drug Taxol[J].Journal of East China University of Science and Technology,1997,23(6):679-683. |
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| Authors: | Wang Hongqiang Zhong Jianjiang Chen Xufeng and Yu Juntang |
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| Institution: | Wang Hongqiang,Zhong Jianjiang *,Chen Xufeng and Yu Juntang |
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| Abstract: | Production of new anticancer drug taxol by plant cell cultures has received an increasing attention.This work studied the kinetics of suspension cultures of Taxus chinensis as our first step of engineering approach to the bioprocess scale up and optimization.The cell growth,utilization of major medium nutrients,oxygen uptake rate,and taxol production were examined on a 250mL flask scale.Our results indicate that the maximum specific growth rate of 0.129/d (based on wet cell weight) was obtained,and the taxol accumulation pattern was found to be mixed growth associated.The maximum production was obtained at the stationary phase.In addition,the effect of addition of V C on the cell cultures was also investigated. |
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| Keywords: | Taxus chinensis taxol production kinetics plant cell culture secondary metabolite |
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