| 由三倍体西瓜下胚轴诱导植株再生的研究 |
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| 引用本文： | 贾勇炯,刘天伦,高茂国,林成鑫,何小莉.由三倍体西瓜下胚轴诱导植株再生的研究[J].四川大学学报(自然科学版),1991(1). |
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| 作者姓名： | 贾勇炯 刘天伦 高茂国 林成鑫 何小莉 |
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| 作者单位： | 四川大学生物系
(贾勇炯，刘天伦，高茂国，林成鑫)，四川大学生物系(何小莉) |
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| 摘 要： | 将三倍体西瓜(Citrullus Vul(?)aris Schrad。)下胚轴切段接种在添加0.02mg/L2,4-D和0.6mg/L6BA的MS培养基上,可诱导出生长旺盛、结构致密的愈伤组织.将愈伤组织转移到含不同浓度的6BA、IAA、IBA和三十烷醇的分化培养基上,分化出了芽和根,进而长成了正常的植株.将不定芽接种在含0.2～0.4mg/L6BA和6mg/L维生素P_1的MS培养基上.可得到许多簇生芽和植物体.用蛭石代替琼脂的生根培养基对于根系的生长和试管苗的移植有良好的效果.经细胞学观察表明,由三倍体西瓜下胚轴诱导出再生植株的染色体倍性有所变化,即有一倍体(n=11)、二倍体(2n=22),三倍体(3n=33)和非整倍体等.
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| 关 键 词： | 三倍体西瓜 下胚轴 再生植株 染色体 |
CALLUS INDUCTION AND PLANTLET REGENERATION FROM HYPOCOTYLS OF TRIPLOID WATER MELON |
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| Jia Yongjiong Liu Tianlun Gai Maoguo Lin Chengxin He Xiaoli.CALLUS INDUCTION AND PLANTLET REGENERATION FROM HYPOCOTYLS OF TRIPLOID WATER MELON[J].Journal of Sichuan University (Natural Science Edition),1991(1). |
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| Authors: | Jia Yongjiong Liu Tianlun Gai Maoguo Lin Chengxin He Xiaoli |
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| Institution: | Department of Biology |
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| Abstract: | The Vigorous and Compact callus was initiated from hypocotyl segments of triploid water melon(Citrullus vulgaris Schrad. )on MS medium supplemented with 0.02mg/L 2,4-D and 0.6mg/L 6BA. Adventitious shoots were produced from Callus by transferring them on MS media with different concentrations of 6BA, IAA, IBA and triaeontanol. Bud clusters could be obtained from advemitious shiuts on MS medium supplemented with 0.3 or 0.4mg/L 6BA and 6mg/L Vitamin E1. Adventitious shoot could be developed roots when culucred on a halt-strength MS medium containing 0.2mg/L IAA and Vermiculite without agar. The number of chromosomes in the root tips cells from regenerateed plant are haploid(n=11), diploid(2n=22) triploid(3n=33) and aneuploid, respectively. |
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| Keywords: | water melon: triploid Callus plantlets of regeneration chromosomes |
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