| 青霉素茵丝体中麦角固醇分离和纯化研究 |
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| 引用本文： | 刘玲,吴正奇,王雄,万端极.青霉素茵丝体中麦角固醇分离和纯化研究[J].湖北大学学报(自然科学版),2013(2):232-235. |
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| 作者姓名： | 刘玲 吴正奇 王雄 万端极 |
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| 作者单位： | 湖北工业大学化学与环境工程学院;湖北工业大学膜技术研究所 |
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| 摘 要： | 探索从青霉素菌丝体中提取麦角固醇以及纯化的方法，并进行条件的优化．实验证明：以麦角固醇含量为1．2％的青霉素湿菌丝为原料，使用浓度为15％的NaOH溶液，物料比1：15，在温度为85℃条件下皂化2h，选择乙醇作为萃取剂进行萃取，是从青霉素菌丝体中提取麦角固醇的最优方法．选择碱性蛋白酶对粗提产品进行酶解，最佳酶解条件为：温度45℃，时间2h，加酶量2．0％，pH为8．在该酶解条件下麦角固醇纯度可达到70％，提取率为95％．
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| 关 键 词： | 菌丝体 碱提 酶解 麦角固醇 |
Study on extraction of ergosterol from penicillin mycelium |
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| LIU Ling,WU Zhengqi,WANG Xiong,WAN Duanji.Study on extraction of ergosterol from penicillin mycelium[J].Journal of Hubei University(Natural Science Edition),2013(2):232-235. |
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| Authors: | LIU Ling WU Zhengqi WANG Xiong WAN Duanji |
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| Institution: | 1.School of Chemical and Environmental Engineering,Hubei University of Technology,Wuhan 430068,China; 2.Institute of Membrane Technology Research,Hubei University of Technology,Wuhan 430068,China) |
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| Abstract: | Penicillium waste mycelium is raw material of penicillin production. In this article, the methods of extract ergosterol from penicillium waste mycelium, were explored and optimized. The penicillin waste mycelium contained 1.2 % ergosteol. Experiments showed the best extraction method. ratio of saponifier, 1 ： 15 ; concentration of sodium hydroxide, 15 % ; the saponification temperature, 85 ℃; reaction time, 2 h. Selected the alkaline protease digested the crude extract. Experiments showed the best hydrolysis condition： the hydrolysis temperature. 45℃; reaction time, 2 h l the amount of enzyme, 2.0%; the hydrolysis pH, 8. Under this condition the purity of ergosterol we got at last could reach 70%, and the extraction rate was 95%. |
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| Keywords: | mycelium alkali extraction enzymatic hydrolysis ergosterol |
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