| 利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率 |
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| 引用本文： | 赵瑾瑜,渠敏,李家璜,张铮,张淑仪,水修基,杨跃涛,华子春.利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率[J].南京大学学报(自然科学版),2011(2):155-163. |
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| 作者姓名： | 赵瑾瑜 渠敏 李家璜 张铮 张淑仪 水修基 杨跃涛 华子春 |
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| 作者单位： | 南京大学声学研究所近代声学实验室;南京大学生命科学院医药生物技术实验室;南京市第一医院;南京大学医学院附属医院; |
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| 基金项目： | 国家自然科学基金(11074125) |
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| 摘 要： | 本文利用脉冲光解法测量了哺乳动物碳氧血红蛋白的光解反应的量子产率.脉冲光解法是利用一束波长为532 nm、脉冲宽度为8 ns、重复频率为10 Hz的脉冲激光照射碳氧血红蛋白溶液,使其发生光解反应.考虑到碳氧血红蛋白溶液和其光解产物脱氧血红蛋白的光吸收系数不同,可利用另一束波长为432 nm的连续激光检测溶液在光解前、后...
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| 关 键 词： | 碳氧血红蛋白 光解反应 量子产率 脉冲激光激发-检测技术 |
Quantum yields in photolyses of mammalian carboxy-hemoglobin studied by pulsed laser pump-probe technique |
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| Zhao Jin-Yu,Qu Min,Li Jia-Huang,Zhang Zheng,Zhang Shu-Yi,Shu Xiu-Ji,Yang Yue-Tao,Hua Zi-Chun.Quantum yields in photolyses of mammalian carboxy-hemoglobin studied by pulsed laser pump-probe technique[J].Journal of Nanjing University: Nat Sci Ed,2011(2):155-163. |
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| Authors: | Zhao Jin-Yu Qu Min Li Jia-Huang Zhang Zheng Zhang Shu-Yi Shu Xiu-Ji Yang Yue-Tao Hua Zi-Chun |
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| Institution: | Zhao Jin-Yu1,Qu Min1,Li Jia-Huang2,Zhang Zheng3,Zhang Shu-Yi1**,Shu Xiu-Ji1,Yang Yue-Tao1,Hua Zi-Chun2(1.Laboratory of Modern Acoustics,Institute of Acoustics,2.Laboratory of Pharmaceutical Biotechnology,Institute of Life Science,3.Nanjing First Hospital,Attached to School of Medicine,Nanjing University,Nanjing,210006,China) |
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| Abstract: | Hemoglobin(Hb) as the allosteric protein,in photo-dissociations of liganded Hb has been studied widely.The mechanisms describing the cooperative binding of CO and other ligands to hemoglobins has been the subject of extensive studies as an important fundamental problem for a long time.In this paper,the quantum yields in photolyses of carboxy-hemoglobins(HbCO) of mammals,such as human,pig,bovine,horse and rabbit,are investigated by the optical pump-probe technique,in which the quantum yield is defined as the... |
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| Keywords: | carboxy-hemoglobin photolysis quantum yield optical pump-probe technique |
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