| 2-TeCD对UVB致NIH3T3细胞损伤的保护作用 |
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| 引用本文： | 尹居鑫,龚平生,佟毅,吴延东,武丽达,吕绍武,闫岗林.2-TeCD对UVB致NIH3T3细胞损伤的保护作用[J].吉林大学学报(理学版),2016,54(6):1451-1455. |
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| 作者姓名： | 尹居鑫 龚平生 佟毅 吴延东 武丽达 吕绍武 闫岗林 |
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| 作者单位： | 1. 吉林大学 分子酶学工程教育部重点实验室, 长春 130012；2. 吉林中粮生化有限公司 玉米深加工国家工程研究中心, 长春 130033；3. 吉林大学 生命科学学院, 长春 130012 |
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| 摘 要： | 采用4,6 二脒基二苯基吲哚（DAPI）荧光染色法和流式细胞术考察谷胱甘肽过氧化物酶（GPx）活力的人工模拟物2-TeCD对UVB致NIH3T3细胞损伤的保护作用. 结果表明： 2-TeCD可影响UVB致NIH3T3细胞中DNA裂解率、 脂质过氧化水平、 活性氧体积分数、 细胞周期及 P53蛋白表达量等指标, 可通过清除自由基发挥对UVB损伤的防护作用.
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| 关 键 词： | 谷胱甘肽过氧化物酶 模拟物 2-TeCD UVB |
| 收稿时间： | 2016-03-08 |
Protective Effect of 2-TeCD on UVB Induced Damage inNIH3T3 Cells |
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| YIN Juxin,GONG Pingsheng,TONG Yi,WU Yandong,WU Lida,LV Shaowu,YAN Ganglin.Protective Effect of 2-TeCD on UVB Induced Damage inNIH3T3 Cells[J].Journal of Jilin University: Sci Ed,2016,54(6):1451-1455. |
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| Authors: | YIN Juxin GONG Pingsheng TONG Yi WU Yandong WU Lida LV Shaowu YAN Ganglin |
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| Institution: | 1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University,Changchun 130012, China; 2. Corn Deep Processing of National Engineering Research Center,Jilin COFCO Biochemical Co., Ltd., Changchun 130033, China;3. College of Life Sciences, Jilin University, Changchun 130012, China |
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| Abstract: | Using fluorescence staining of 4,6 diamidino 2 phenylindole dihyodrochloride and flow cytometry we studied protective effect of an artificial mimic 2 tellurium bridged cyclodextrin (2-TeCD) with the activity of glutathione peroxidase (GPx) on UVB induced damage in NIH3T3 cells. The results show that 2 TeCD can affect DNA lysis rate, the level of lipid peroxidation,the volume fraction of ROS, cell cycle, P53 protein expression and other indicators in NIH3T3 cells caused by UVB. The 2 TeCD can play a protective role against UVB damage by removing free radicals. |
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| Keywords: | UVB gluathione peroxidase (GPx) mimic 2-TeCD |
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