| 双抗体夹心ELISA法测定人凝血因子IX蛋白 |
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| 引用本文： | 戴一凡,刘坚,邱信芳,薛京伦.双抗体夹心ELISA法测定人凝血因子IX蛋白[J].复旦学报(自然科学版),1990(4). |
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| 作者姓名： | 戴一凡 刘坚 邱信芳 薛京伦 |
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| 作者单位： | 复旦大学遗传学研究所
(戴一凡，刘坚，邱信芳)，复旦大学遗传学研究所(薛京伦) |
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| 摘 要： | 用双抗体夹心ELISA法测定人凝血因子IX蛋白。在转入了外源人凝血因子IX基因的CHO细胞和HSF细胞的培养液中均测得一定量的IX因子蛋白,其量相当于正常人血浆IX因子蛋白的0.89%～3.81%,而对照细胞均为阴性结果。对一例血友病B患者(IX:C<0.1%)的分析结果表明,其血浆中含有的IX因子蛋白量相当于正常人的52.3%,因而将此病例归属于血友病B~R型。本法灵敏度高、特异性强、重复性好,可与一期法相互补充,应用于临床血友病B的诊断、分类、家系分析及携带者的检出。
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| 关 键 词： | 血友病B 凝血因子IX 培养介质 ELISA |
DETECTION OF HUMAN CLOTTING FACTOR IX ANTIGEN BY A DOUBLE ANTIBODY ELISA |
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| Dai Yifan,Liu Jian,Qiu Xinfang,Xue Jinglun,.DETECTION OF HUMAN CLOTTING FACTOR IX ANTIGEN BY A DOUBLE ANTIBODY ELISA[J].Journal of Fudan University(Natural Science),1990(4). |
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| Authors: | Dai Yifan Liu Jian Qiu Xinfang Xue Jinglun |
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| Institution: | Institute of Genetics |
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| Abstract: | A modified double antibody ELISA was used to monitor the human factor IXantigen synthesis in the human FIX cDNA transfected CHO and HSF (humanskin fibroblast) cells. The results showed that these transfected cells can produceFIX antigen at the level of 0.89%~3.81% of pooled normal human plasma, whilethe controls were negative. This assay was also used to analyze the plasma from ahemophilia B patient, and a 52.3% amount of factor IX antigen was found as com-pared to normal plasma. Thus the patient could be classified as the hemophilia B~Rtype. Complementary to the one-stage method used in the clinics, this assay can bepromising in the diagnosis and classification of hemophilia B patients, the pedi-gree analysis and detection of carriers. |
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| Keywords: | hemophilia B factor IX culture media ELISA |
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