| 蔬菜中硝酸盐和亚硝酸盐的不同提取方法及含量测定 |
| |
| 引用本文： | 陈秋生,梁淑轩,李科,孙汉文.蔬菜中硝酸盐和亚硝酸盐的不同提取方法及含量测定[J].河北大学学报(自然科学版),2007,27(2):174-178. |
| |
| 作者姓名： | 陈秋生 梁淑轩 李科 孙汉文 |
| |
| 作者单位： | 河北大学,化不与环境科学学院,河北省分析科学与技术重点实验室,河北,保定,071002 |
| |
| 基金项目： | 河北大学博士基金资助项目(NO.026) |
| |
| 摘 要： | 对超声提取、振荡提取、热水提取等不同的样品提取技术进行比较,选择用简便高效的超声提取技术提取蔬菜中的硝酸盐和亚硝酸盐.用N-(1-萘基)-乙二胺分光光度法测定亚硝酸盐含量,用镉柱还原法测定硝酸盐含量.NO2-和NO3-的回收率分别为 97.5%～100.2%和96.8%～103.7%,RSD分别为6.05%和0.24%.
|
| 关 键 词： | 蔬菜 硝酸盐 亚硝酸盐 提取 |
| 文章编号： | 1000-1565(2007)02-0174-05 |
| 修稿时间： | 2006年5月14日 |
Comparison of Several Extraction Methods for the Spectrophotometric Determination of Nitrate and Nitrite in Vegetables |
| |
| CHEN Qiu-sheng,LIANG Shu-xuan,LI Ke,SUN Han-wen.Comparison of Several Extraction Methods for the Spectrophotometric Determination of Nitrate and Nitrite in Vegetables[J].Journal of Hebei University (Natural Science Edition),2007,27(2):174-178. |
| |
| Authors: | CHEN Qiu-sheng LIANG Shu-xuan LI Ke SUN Han-wen |
| |
| Abstract: | A comparative study on three pretreatment methods,ultrasonic extraction method,shaking extraction method,and hot water stationary soaking extraction method,for the spectrophotometric determination of nitrate and nitrite in vegetable has been carried out in this paper.The results suggested ultrasonic extraction was fast and efficient.Nitrite reacts with N-(1-naphthyl) ethylenediamine to form water soluble reddish violent dye whose maximum absorptive wavelength is 540nm.Nitrate was reduced by a cadmium column,then determined by the same method as nitrite.The recovery of NO_2-and NO_3-was 97.5%～100.2% and 96.8%～103.7% respectively.RSD was 6.05% for nitrate and 0.24% for nitrite.The contents of NO_2-and NO_3-in different kinds of vegetables were determined by the suggested method. |
| |
| Keywords: | ultrasonic extraction nitrate nitrite vegetables |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《河北大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《河北大学学报(自然科学版)》下载免费的PDF全文 |
