| ApoE基因敲除小鼠和C57BL/6J小鼠血脂及主动脉组织病理学的对比观察 |
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| 引用本文： | 程军,李金平,田卓,胡厚源.ApoE基因敲除小鼠和C57BL/6J小鼠血脂及主动脉组织病理学的对比观察[J].实验动物科学,2008,25(2):4-6. |
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| 作者姓名： | 程军 李金平 田卓 胡厚源 |
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| 作者单位： | 第三军医大学西南医院心内科,重庆,400038 |
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| 摘 要： | 目的 高脂饲养的雄性载脂蛋白E基因缺陷小鼠动脉粥样硬化模型的建立。方法 选取8周龄雄性ApoE-/-小鼠,以C57BL/6J小鼠为对照组,高脂饲养8周后测定血清中总胆固醇、甘油三酯、高密度胆固醇脂蛋白、低密度胆固醇脂蛋白含量;小鼠心脏主动脉根部石蜡切片,常规HE染色,观察和测量动脉粥样硬化斑块的组织学特点及病变面积。结果 ApoE-/-小鼠血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇的含量显著高于同年龄同性别C57BL/6J小鼠,高密度脂蛋白的含量显著低于同龄同性别C57BL/6J小鼠;高脂饲养下16周龄ApoE-/-小鼠主动脉根部切面可见明显的脂质斑块。结论 在该浓度的高脂饲料饲养条件下,ApoE-/-小鼠在16周龄已形成脂质斑块,是研究动脉粥样硬化等心血管疾病较佳的动物模型。
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| 关 键 词： | 载脂蛋白E基因缺陷小鼠 动脉粥样硬化 血脂 |
| 文章编号： | 1006-6179(2008)02-0004-03 |
| 修稿时间： | 2007年11月12 |
Observation on Blood-lipoid and Histopathological Changes of Aortic Root in Apolipoprotein-EKnockout Mice and C57BL/6J Mice Fed with Feed Rich in Fat |
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| CHENG Jun,LI Jin-ping,TIAN Zhuo,HU Hou-yuan.Observation on Blood-lipoid and Histopathological Changes of Aortic Root in Apolipoprotein-EKnockout Mice and C57BL/6J Mice Fed with Feed Rich in Fat[J].Shiyan Dongwu Kexue,2008,25(2):4-6. |
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| Authors: | CHENG Jun LI Jin-ping TIAN Zhuo HU Hou-yuan |
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| Institution: | CHENG Jun,LI Jin-ping,TIAN Zhuo,HU Hou-yuan(Department of Cardiology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To develop atherosclerotic model of apolipoprotein-E(ApoE) knockout mice.Methods Six male 8-week-old ApoE-knockout mice were fed with atherogenic diet for 8 weeks,and 6 male 8-week-old C57BL/6J mice were used as controls.The mice were sacrificed,and blood was drawn for determination of ssubjected to paraffin sections stained with hematoxylin and eosine to examine atherosclerotic lesions in aortic root.Results Serum TG,TC,and LDL-C levels in ApoE-knockout mice were significantly higher than those ... |
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| Keywords: | ApoE-knockout mice Arteriosclerosis Blood lipid |
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