| 不同化学激活方法对小鼠卵母细胞孤雌激活效果的影响 |
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| 引用本文： | 张玉影,曾晓辉,付晓兰,李雪峰.不同化学激活方法对小鼠卵母细胞孤雌激活效果的影响[J].华南师范大学学报(自然科学版),2010(1):98-104. |
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| 作者姓名： | 张玉影 曾晓辉 付晓兰 李雪峰 |
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| 作者单位： | 华南师范大学生命科学学院,广东广州,510631 |
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| 基金项目： | 国家自然科学基金资助项目(30370724) |
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| 摘 要： | 通过比较几种常用的化学激活方法对小鼠卵母细胞的孤雌激活和孤雌生殖胚胎体外发育的影响,从而筛选出小鼠卵母细胞孤雌激活的适宜方法.选取成熟小鼠卵母细胞,随机分为以下9组进行激活处理:①离子霉素(I)+6-二甲氨基嘌呤(D);②I+D+细胞松弛素B(CB);③I+放线菌酮(C);④I+C+CB;⑤φ(乙醇)=7%的乙醇(E)+D;⑥E+D+CB;⑦E+C;⑧E+C+CB;⑨Sr2++CB.比较了这9种化学激活方法以及SrCl2的作用时间和卵龄对小鼠卵母细胞激活效果的影响.实验结果表明:小鼠卵母细胞孤雌激活率与激活措施和卵龄有关;小鼠卵母细胞可以被I+D+CB或SrCl2+CB有效激活,激活率及孵化囊胚发育率均高于其他激活方法(P0.05),且以SrCl2+CB最为有效.hCG注射后20h为小鼠卵母细胞进行SrCl2激活的最适卵龄,其最佳处理时间为4h.对小鼠卵母细胞而言,6-DMAP比放线菌酮的激活效果好.
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| 关 键 词： | 卵母细胞 化学激活 激活方法 小鼠 |
EFFECTS OF DIFFERENT CHEMICAL ACTIVATIONS ON THE DEVELOPMENT OF MOUSE PARTHENOGENETIC EMBRYOS |
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| ZHANG Yuying,ZENG Xiaohui,FU Xiaolan,LI Xuefeng.EFFECTS OF DIFFERENT CHEMICAL ACTIVATIONS ON THE DEVELOPMENT OF MOUSE PARTHENOGENETIC EMBRYOS[J].Journal of South China Normal University(Natural Science Edition),2010(1):98-104. |
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| Authors: | ZHANG Yuying ZENG Xiaohui FU Xiaolan LI Xuefeng |
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| Institution: | School of Life Science;South China Normal University;Guangzhou 510631;China |
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| Abstract: | In order to find out the suitable activation protocol for mouse oocytes, the cleavage and development of mouse oocytes activated by several different chemical activation protocols were investigated. Matured oocytes were grouped into 9 treatments randomly: (1) inomycin + 6-dimethylaminopurine; (2) ionomycin + 6-DMAP+ cytochalasin B (CCB); (3) ionomycin+ cycloheximide (CHX); (4) ionomycin + CHX + CCB; (5) 7% ethanol + 6-DMAP; (6) 7% ethanol + 6-DMAP + CCB; (7) 7% ethanol + CHX; (8) 7% ethanol + CHX + CCB; (9)... |
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| Keywords: | oocytes chemical activation activation protocol mouse |
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