| 抗人FXYD6单克隆抗体的制备及鉴定 |
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| 引用本文： | 刘军桂?,周宁新.抗人FXYD6单克隆抗体的制备及鉴定[J].科学技术与工程,2009,9(10). |
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| 作者姓名： | 刘军桂? 周宁新 |
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| 作者单位： | 第二炮兵总医院肝胆胃肠病研究所,北京,100088 |
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| 摘 要： | 制备了抗人FXYD6单克隆抗体,并进行初步鉴定.以FXYD6合成多肽作为免疫原,利用单克隆抗体杂交瘤技术建立阳性克隆细胞株;腹水诱导法制备抗人FXYD6单克隆抗体;蛋白A亲合层析法纯化;ELISA测定FXYD6单克隆抗体的特异性和效价;以所得特异性抗体为一抗,利用免疫组化法检测胰腺癌组织中FXYD6的表达.结果成功获得1株稳定分泌特异性抗人FXYD6单克隆抗体的杂交瘤细胞株;腹水诱导法生产抗人FXYD6单克隆抗体2.86 mg;FXYD6单克隆抗体可以与FXYD6合成多肽特异性结合,抗体效价1:5400;免疫组织化学显示胰腺癌组织中胞膜呈阳性染色.成功获得FXYD6单克隆抗体可以为进一步研究FXYD6的组织分布、生物学作用创造条件.
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| 关 键 词： | 离子通道转运调节因子 单克隆抗体 酶联免疫吸附测定 免疫组织化学 |
Preparation and Identification of Monoclonal Antibodies Against FXYD6 |
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| LIU Jun-gui,ZHOU Ning-xin,ZHANG Xiao-dong.Preparation and Identification of Monoclonal Antibodies Against FXYD6[J].Science Technology and Engineering,2009,9(10). |
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| Authors: | LIU Jun-gui ZHOU Ning-xin ZHANG Xiao-dong |
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| Institution: | Institute of Hepatobiliary and Gastrointestinal Surgery;General Hospital of the Second Artillery;Beijing 100088;P.R. China |
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| Abstract: | To prepare and identify anti-human monoclonal antibody(McAb)against FXYD6. Hybridoma cell line which could secrete anti-human McAb against FXYD6 was established by hybridoma technique. Anti-human McAb against FXYD6 was produced by ascites revulsion. Purified McAb was prepared by protein A chromatography. Titer and specificity of McAb were assessed by ELISA. Expression of FXYD6 in pancreatic cancer was detected by Immunohistochemistry. One hybridoma cell line that can secrete anti-human FXYD6 McAb was establ... |
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| Keywords: | FXYD6 |
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