鲜艾叶与干艾叶挥发性成分的HS-SPME-GC-MS分析

采用顶空固相微萃取-气相色谱质谱联用（HS-SPME-GC-MS）技术分析比较鲜艾叶与干艾叶的挥发性成分。考察了不同萃取头和萃取温度对艾叶挥发性成分的萃取效果，以选用65 μm PDMS/DVB SPME萃取头和60 ℃水浴加热时萃取效果最好；采用峰面积归一化法测定各成分的质量分数，共鉴定出58种挥发性成分，主要为烯烃类、芳香类和醇类物质，占总峰面积90%以上。鲜艾叶与干艾叶均含有莰烯、1-辛烯-3-醇、α-水芹烯、邻聚伞花素、桉油精、石竹烯等，此外又各自具有特有成分，表明艾叶在干制后其挥发性成分发生变化。

HS-SPME-GC-MS analysis of volatile components in fresh and dried leaves of Artemisia argyi

We analyzed volatile components in fresh and dried leaves of Artemisia argyi Levl.et Vant.with headspace solid phase microextraction（SPME） and GC-MS.We also investigated the impacts of different SPME fibers and extraction temperature on the extracts.Results demonstrate that 65 μm PDMS/DVB fibre and temperature of 60 ℃ exhibit good extraction effect.We employed peak area normalization method to determine the volatile components.Fifty-eight compounds are identified in the fresh and dried leaves of Artemisia argyi,which include alkanes,alkenes and alcohols,and whose area exceeds 90% of the total peak area.Results also show that fresh and dried leaves of Artemisia argyi not only contain identical volatile compounds such as Camphene,1-Octen-3-ol,alpha-Phellandrene,Benzene,1-methyl-2-（1-methylethyl）-Eucalyptol and Caryophyllene,but also contain their own unique volatile compounds.This shows that their volatile components change after dryness.