| 从发酵液中直接提取柚苷酶 |
| |
| 引用本文： | 游见明,吕开斌.从发酵液中直接提取柚苷酶[J].四川理工学院学报(自然科学版),2009,22(1). |
| |
| 作者姓名： | 游见明 吕开斌 |
| |
| 作者单位： | 四川理工学院生物工程学院,四川,自贡,643000 |
| |
| 基金项目： | 四川省科学技术厅重点科技计划 |
| |
| 摘 要： | 用双水相萃取方法直接从发酵液中提取柚苷酶进行了实验,就聚乙二醇-硫酸铵双水相系统中的聚乙二醇相对分子量、聚乙二醇浓度、硫酸铵浓度、无机盐的添加等因素对柚苷酶的分配行为的影响进行了探索。结果显示:在用浓度为14%~16%的PEG1000与浓度为14%~18%硫酸铵和0.1%氯化钠组成的双水相体系中,柚苷酶的分配系数达1.85以上,酶活力回收率达80%,提纯倍数为2.1。
|
| 关 键 词： | 柚苷酶 双水相系统 萃取 聚乙二醇-硫酸铵 |
Direct Extraction of Naringinase From Fermentation Liquor |
| |
| YOU Jian-ming,LV Kai-bin.Direct Extraction of Naringinase From Fermentation Liquor[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2009,22(1). |
| |
| Authors: | YOU Jian-ming LV Kai-bin |
| |
| Institution: | School of Biotechnology Engineering;Sichuan University of Science & Engineering;Zigong 643000;China |
| |
| Abstract: | The naringinase was extracted by aqueous two-phase from fermentation liquor.The effect produced by the distributing of naringinase that was caused by such factors as the relative molecular weight of PEG,the consistency of PEG,the consistency of ammonium sulfate and the appending of mineral salt in the aqueous two-phase system of PEG-ammonium sulfate was explored.The result showed that the coefficient of naringinase was more than 1.85,the recovery rate of enzyme activity was up to 80% and the purifying multi... |
| |
| Keywords: | naringinase aqueous two-phase system extraction PEG-ammonium sulfate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
