利用二甲基黄的氧化褪色反应测定食物的硒含量

在酸性条件下，硒（ＩＶ）能催化氨酸钾氧化二甲基黄使之褪色，反应溶液中含硒（ＩＶ）量与ｌｇ（Ａｏ／Ａ）在一定范围内呈良好的线性关系；（Ａｏ和Ａ分别为非催化反应和催化反应溶液的吸光度）以此二点测定食物的硒含量获得了较满意的结果。本方法具有灵敏度高、专一性强、无需分离或富集、快速简便等特点。其硒含量的线性范围为２－２００ｎｇ／ｍｌ。

Using Oxidiation and Fading of Dimethyl Yellow for Determination of Selenium in Food

The selenium(IV) can catalyze oxidation reaction of the dimethyl yellow with potassium chlorate under acidic conditionl and the dimethyl yellow fading. And the content of selenium(IV) versus lg (A./A) shows good linear relationship on the definite range (A0: absorba ace of the solution of the non-catalytic reaction. A: absorbance of the solution of the catalytic reaction). According to these,the good results of the determnation of the determination of selenium are obtained. The method possesses the characteristics of high sensitivity, strong selectivity, unnecessary separation and enrichment. The linear range of the selenium(IV) content is 2-200ng/ml.