葛根素对海洛因依赖大鼠心脏功能的影响及机制

目的:观察葛根素对海洛因依赖大鼠戒断期间心脏功能的变化;并探讨其与一氧化氮(NO)/一氧化氮合酶(NOS)的关系。方法:采用剂量递增法建立海洛因成瘾动物模型,共15 d,最后一次注射海洛因后腹腔注射纳洛酮并观察戒断症状。第17天,利用PowLab计算机生物信号记录分析系统测定大鼠心率(HR)、平均动脉压(mAP);并且通过测定左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、左心室内压最大上升或下降速率(±dp/dtmax);采用比色法测定大鼠心肌组织匀浆中iNOS(诱导型一氧化氮合酶)活力及NO含量。结果:葛根素预处理组与heroin组比较,大鼠HR加快,mAP降低;LVSP升高、LVEDP降低、±dp/dtmax均有所升高;心肌组织iNOS的活力降低,NO的含量减少。结论:葛根素能改善海洛因对心脏功能的抑制作用,其作用与一氧化氮(NO)/一氧化氮合酶(NOS)体系有关。

Effect and Mechanism of Puerarin on Cardiac Function of Heroin Dependence Rats

Objective： To evaluate the effect and mechanism of Puerarin on the cardiac function of heroin dependence rats. Methods： The heroin dependence models were established by a 15-day increasing dose of heroin intraperitoneal injection in rats, and then rats were withdrawn by naloxone. Withdrawal symptoms were used to evaluate the model establishment. On the 17th day, the mean arterial pressure （mAP）, heart rate （HR）, left ventricular systolic pressure （LVSP）, left ventricular end-diastolic pressure （LVEDP） and left ventrieular pressure development during isovolumetric contraction/relaxation （＋dp/dtmax） were monitored. The level of nitric oxide （NO） and inducible nitric oxide synthase （iNOS） of cardiac muscle were detected by the colorimetric technique method. Results： Compared with heroin group, mAP and LVEDP in heroin ＋ Puerarin group decreased, while HR, LVSP and ＋dp/dtmax in heroin ＋ Puerarin group increased. NO and iNOS in cardiac muscle were significantly decreased in heroin ＋ Puerarin group. Conclusion： Puerarin could improve myocardial function in heroin dependence rats, which may relate to the decreased concentration of myocardial NO and NOS.