利用Brevibacillus laterosporus YMF3.00003菌株研制生物杀菌剂

 Brevibacillus laterosporus YMF3.00003菌株具有广谱抗真菌活性,摇瓶培养发酵液对所测的植物病原菌Cylindrocarpon didynum,C.destructans,Magnaporthe grisea,Rhizoctonia solani的菌丝生长有较强的抑制作用,最强抑制率分别为81.5%,71.0%,60.7%和60.2%.确定了该菌株在20 L发酵罐中的发酵条件,绘制了生长曲线及得到了不同生长时期的抑菌率.其中,培养21 h时细胞数最多,达到5.1×109;25 h时发酵物对R.solani的抑菌率最高,达到90.5%.通过对几种吸附剂的筛选,确定用锯木屑作为吸附剂,将发酵终产物用锯木屑吸附,制成约2×10¹¹g^-1的菌剂.

Development of a formulation against phytopathogens using Brevibacillus laterosporus YMF3. 00003 strain

Brevibacillus laterosporus YMF3.00003 strain,can effectively inhibit the mycelial growth of most phytopathogens.The highest growth inhibition rates of the liquid culture of YMF3.00003 against four tested phytopathogens,Cylindrocarpon didynum,C.destructans,Magnaporthegrisea and Rhizoctonia solani,were 81.5%,71.0%,60.7% and 60.2% respectively.In 20L biologic reaction jar,the fermentation conditions,growth curve and fungi static dynamics of the strain were determined and presented.In this jar,the highest cell number,5.1?10⁹,was obtained after incubation of 21 hours and the culture showed the highest inhibition(90.5%) to mycelialgrowth of R.solani at 25hours after inoculation.Furthermore,sawdust was selected out of 7 tested candidates as the sorbent for formulation development and a biocontrol agent which containing about 2?10¹¹ cells/g wasdeveloped.