| 赤潮甲藻分类学方法研究（一）─—有壳甲藻甲片的解离、染色及观察 |
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| 引用本文： | 钱锋,齐雨藻,朱从举.赤潮甲藻分类学方法研究（一）─—有壳甲藻甲片的解离、染色及观察[J].暨南大学学报,1995(1). |
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| 作者姓名： | 钱锋 齐雨藻 朱从举 |
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| 作者单位： | 暨南大学水生生物研究所 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 比较研究了有壳甲藻甲片的处理和观察方法，包括２种甲片分离法：壳壁剥离法和原生质去除法；５种甲片染色法：锥虫蓝法、固绿法、Ｉ＿２－ＨＩ－水合氯醛法、Ｉ＿２－ＫＩ－水合氯醛法和荧光染色法。发现用荧光染色和经壳壁剥离法处理后用Ｉ＿２－ＫＩ－水合氯醛染色液染色的样品，观察效果最好。
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| 关 键 词： | 甲藻 分类学 甲藻甲片 |
Methodological Studies on Dinoflagellate Taxonomy |
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| I.Thecal Isolation and Stainning Qian Feng,Qi Yuzao,Zhu Congji.Methodological Studies on Dinoflagellate Taxonomy[J].Journal of Jinan University(Natural Science & Medicine Edition),1995(1). |
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| Authors: | IThecal Isolation and Stainning Qian Feng Qi Yuzao Zhu Congji |
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| Abstract: | A comparation study is conducted on threatments and light-microscope observation of ar-mored dinoflagellates.2 methods for thecal plates.dissection,theal dissection and protoplastextraction,are introduced,and the following 5 different ways of theal stainning,usingseparately trypan blue,fast green,HI/I_2/chloral hydrate,KI/I_2/chloral hydrate and calcofluorwhite,are applied.It rasults that calcofluor White and KI/I_2/chloral hydrate are the two bestways to serve the purpose. |
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| Keywords: | dinoflagellate taxonomy thecal plate |
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