| 野生二粒小麦高分子量谷蛋白亚基组成的多态性研究 |
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| 引用本文： | 张大乐,李黎,方圆,苏亚蕊,李玉阁,李锁平.野生二粒小麦高分子量谷蛋白亚基组成的多态性研究[J].河南大学学报(自然科学版),2012,42(3):286-290. |
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| 作者姓名： | 张大乐 李黎 方圆 苏亚蕊 李玉阁 李锁平 |
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| 作者单位： | 河南大学生命科学学院,河南开封,475004 |
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| 基金项目： | 国家自然科学基金项目，“十二五”农村领域国家科技计划课题，河南省教育厅自然科学研究项目 |
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| 摘 要： | 对来自以色列的38份野生二粒小麦的高分子量谷蛋白亚基(high molecular weight glutenin subunits,HMW-GS)组成进行SDS-PAGE检测,发现其HMW-GS具有较高的遗传多样性.在Glu-A1和Glu-B1位点共有18种可确定的亚基变异类型.其中,A1位点上的亚基1、2*(出现频率分别为60.53%和13.16%)和B1位点上的亚基13+16、14+15与17+18为优质亚基(出现频率分别为26.32%、7.89%和2.63%).同时出现了许多普通小麦所稀有或缺乏的特异亚基类型,如6+8*,7*+9,6+9*,7+9*,20,22等,在2份材料中出现了1种未命名的新亚基类型.
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| 关 键 词： | 野生二粒小麦 高分子量谷蛋白亚基 SDS-PAGE |
Diversity Study on the Subunit Composition of High Molecular Weight Glutenin in Wild Emmer |
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| ZHANG Da-le
,
LI Li
,
FANG Yuan
,
SU Ya-rui
,
LI Yu-ge
,
LI Suo-ping.Diversity Study on the Subunit Composition of High Molecular Weight Glutenin in Wild Emmer[J].Journal of Henan University(Natural Science),2012,42(3):286-290. |
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| Authors: | ZHANG Da-le LI Li FANG Yuan SU Ya-rui LI Yu-ge LI Suo-ping |
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| Institution: | *(College of Life Science,Henan University,Henan Kaifeng 475004,China) |
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| Abstract: | SDS-PAGE detection was conducted on 38 shares of wild emmer from Israel and it was found that their high molecular weight glutenin subunits(HMW-GS) had higher genetic diversity.18 types of confirmable subunit variations appeared on the loci of Glu-A1 and Glu-B1.Among them,subunits 1 and 2*(with occurrence frequencies 60.53% and 13.16%) on A1 locus and subunits 13+16,14+15 and 17+18(with occurrence frequencies 26.32%,7.89% and 2.63%,respectively) on B1 locus were high-quality subunits.In addition,many peculiar subunit types rare in common wheat were found,such as 6+8*,7*+9,6+9*,7+9*,20,22,etc.And one new unnamed subunit type occurred in two shares of materials. |
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| Keywords: | wild emmer HMW-GS SDS-PAGE |
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