Cloning and characterization of vernalization-related gene (ver203F) cDNA 3' end |
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| Institution: | YONG Weidong, CHONG Kang , LIANG Tiebing, XU Zhihong , TAN Kehui and ZHU Zhiqing
Research Center for Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences , Beijing 100093, China ;
Shanghai Institute of Plant Physiology , Chinese Academy of Sciences , Shanghai 200032, China Corresponding authors |
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| Abstract: | Based on the cDNA fragment sequence of vernalization-related gene verc203 cloned by differential screening in our lab, the 5' primer has been designed. The cDNA 3' end of ver203 gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the gene has homology with Hordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate. |
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| Keywords: | wheat vernalization-related gene cDNA 3' end RACE |
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